土茯苓中药复方制剂与大蒜油对小鼠的排铅作用比较

Comparison Study of the Effect of Lead Excretion of Smilax Glabra and Garlic Oil on Mice

目的:探讨土茯苓中药复方制剂和大蒜油的排铅效果,为铅中毒的防治提供一定的理论依据。方法:以40 mg/L的醋酸铅溶液,按0.01 mL/g体重剂量连续15 d灌胃造模,造模成功后将48只雄性成年小鼠随机分成4组,即土茯苓中药复方制剂组、大蒜油组、乙二胺四乙酸组和中毒不治疗组,连续15 d驱铅治疗。在造模前、第16天、第31天分别测定各组小鼠的体重及抽取小鼠尾部全血并用石墨炉原子吸收法直接测定小鼠的血铅含量,对实验结果进行统计学分析。结果:①血铅含量:统计学意义驱铅治疗后,土茯苓中药复方制剂组、大蒜油组、乙二胺四乙酸组分别与中毒不治疗组的血铅含量比较,差异均有统计学意义(P<0.01);土茯苓中药复方制剂组、大蒜油组、乙二胺四乙酸组的血铅含量两两比较,差异有统计学意义(P<0.01)。驱铅治疗后各组的血铅含量为:土茯苓中药复方制剂组<EDTA组<大蒜油组<中毒不治疗组。②体重:土茯苓中药复方制剂组、大蒜油组分别与中毒不治疗组的体重比较,差异有统计学意义(P<0.05);土茯苓中药复方制剂组与大蒜油组的体重比较,差异有统计学意义(P<0.05)。土茯苓中药复方制剂组的体重>大蒜油组的体重。③存活率:土茯苓中药复方制剂组、大蒜油组分别与中毒不治疗组的存活率比较,差异有统计学意义(P<0.01);土茯苓中药复方制剂组与大蒜油组的存活率比较,差异无统计学意义(P>0.05)。结论:土茯苓中药复方制剂、大蒜油对血铅均有促排作用;驱铅功效为:土茯苓中药复方制剂>大蒜油。

Objective： To compare the lead excretion effect of smilax glabra and garlic oil, and provide the theoretical evidence to prevent lead poisoning. Methods： Lead poisoning model was constructed by lead acetate solution （40 mg/L） lavage at the standard of 0.01 mL/g weight for 15 days. After the lead exposure, 48 mice were randomly divided into 4 groups treated with smilax glabra, garlic oil, EDTA and distilled water respectively for 15 days. Mice weights and lead concentrations were recorded before the lead poisoning, the 16th day and the 31st day. Lead concentrations of tail vein blood were determined by the method of graphite furnace atomic absorption. Results： （~）With different treatment, there were significant differences on the lead content in the blood among smilax glabra group, EDTA group, garlic oil group and distilled water group （P〈0.01）. After the therapy of the lead excreation, the blood lead content in smilax glabra group〈EDTA group〈garlic oil group〈 distilled water group. Compared to the distilled water group, there were both significant differences in weight of smilax glabra group and garlic oil group （P〈0.05）; the weight of the smilax group was heavier than that of the garlic oil group （/9〈0.05）. （~）Compared to the distilled water group, there were both significant differences in survival rate of smilax glabra group and garlic oil group （P〈0.05）; there was no statistical difference on the survival rate of the smilax glabra group and garlic oil group （P〉0.05）. Conclusion： Smilax glabra and garlic oil can promote the excretion of lead, our data showed better lead excretion effect of Smilax glabra compared to the garlic oil.