不同酶制剂水平对北京填鸭生长性能、血浆生化指标和肝脏组织学的影响

Effects of Supplementing Different Levels of a Commercial Enzyme Complex on Growth Performance,Plasma Biochemical Parameters and Liver Histology for Pekin Ducks by Overfeeding

为了研究不同酶制剂水平对北京填鸭生长性能、血浆生化指标及肝脏组织学的影响。选取35日龄健康、大小均匀的Z4系雄性北京鸭96只,随机分成4个处理,每个处理6个重复,每个重复4只鸭。对照组填饲基础日粮,试验1,2和3组在基础日粮中分别添加100,200,300 mg/kg的复合酶制剂,试验鸭在35,36日龄填饲量分别为260,300 g,之后5 d各处理填饲量均为400 g/d,试验期为7 d。结果表明,200,300 mg/kg酶组日增质量(ADG)和料质量比(F/G)显著优于对照组和100 mg/kg酶组(P<0.05),100 mg/kg酶组与对照组无显著差异(P>0.05),200,300mg/kg酶组间无显著差异(P>0.05);不同酶制剂水平对北京填鸭血浆葡萄糖(Glu)、甘油三酯(TG)、高密度脂蛋白(HDL)的浓度影响不大(P>0.05),而对血浆总胆固醇(CHO)和低密度脂蛋白(LDL)的浓度有显著影响作用(P<0.05)。各组北京填鸭肝脏细胞肿大,看不清完整的细胞形态,胞浆中充满大量大小不等的脂肪滴,将细胞核挤压到一侧,添加酶制剂能够增加肝脏内脂肪的沉积量。综上所示,酶制剂在一定范围内能够提高北京填鸭日增质量,降低料质量比,当添加量超过一定水平时,其作用效果不明显。

This experiment was conducted to determine the effects of different levels of a commercial enzyme complex on growth performance,plasma biochemical parameters and liver histology for Pekin ducks by overfeeding.Ninety-six 35-day-old health male Pekin ducks with similar body weight were randomly assigned into 4 treatments with 6 replicates per treatment and 4 Pekin ducks per replicate.The corn-soybean diet was provided to the four groups of ducks：The control diet and the other three enzyme complex supplemented diets which were 100,200,300 mg/kg enzyme complex supplemented to the control diet,respectively.The feed intake of four treatment groups in the first 2 days was the same in 260 g/bird at 35 d and 300 g/bird at 36 d of age,and then the amount of feed provided to the ducks in each treatment remained unchanging during the other 5 days and was 400 g/（d · bird）,for each treatment group respectively.The experimental feeding period lasted 7 d from 35 to 42 d of age.The results showed that：Compared with the control group and the group of 100 mg/kg enzyme complex,the Pekin ducks overfed the diets supplemented with 200,300 mg/kg enzyme complex had better ADG and F/G（P0.05）.However,growth performance was no significant differences between the group of 100 mg/kg enzyme complex and the control group（P0.05）,the 200 mg/kg enzyme complex group and the 300 mg/kg enzyme complex group（P0.05）.No significant differences in plasma Glu,TG,HDL levels were observed among treatments（P0.05）while plasma CHO and LDL levels had significant differences（P0.05）.Histology observation showed that swelling of hepatocyte were observed and the cytoplasm was full of fat in each group.No whole cell shape was observed and the cell nuclei are squeezed to one side.Enzyme can increase fat deposition of hepatocyte in force-feeding ducks.In conclusion,supplementation of enzyme complex was beneficial for overfed Pekin ducks,while excess enzyme complex can not induce a better performance.