重组几丁质酶对采后葡萄贮藏过程中抗病效果的研究

Effect of the Recombinant Chitinase on the Disease Resistance in Table Grape during Postharvest Storage

以克瑞森无核葡萄品种为试材,采用重组几丁质酶对接种灰霉菌的葡萄果实进行处理,研究重组几丁质酶对葡萄果实腐烂的抑制及其诱导抗病作用。结果表明:在25℃条件下,重组几丁质酶处理能诱导采后葡萄的抗病性,不仅增加了葡萄果实中POD、PPO和PAL的酶活性,而且降低了丙二醛含量,诱导了果实中酚类物质含量的积累;在不同程度上延缓了葡萄的发病时间,降低了发病指数,贮藏至第6天时,对照的发病指数已达到100%,而重组几丁质酶处理的发病指数仅为47.8%。总的来看,重组几丁质酶处理可以提高葡萄采后抗病性,在贮藏初期抑制由灰霉引起的葡萄腐烂。

The objective of this study was to determine the mechanism and disease-resistant effect of recominant chitinase on the table grape during storage.The purified recombinant chitinase was added to the wound of Crimson seedless table grape which had already been inoculated with Botrytis cinerea.The activity of disease-resistant enzyme of the treated grape was analyzed during storage at 25℃.The results showed that the recombinant chitinase treatment could induce the increase of POD,PPO and PAL enzyme activity,and reduce the content of MDA and induce fruit phenolics content accumulation.Recombinant chitinase could retard onset time of grey disease symptom and lower disease index during grape storage.Morbidity index of control grape reached 100% on day 6,but recombinant chitinase treated grape only had the mordity index of 47.8%.Overall,the recombinant chitinase could induce disease-resistance of grape to Botrytis cinerea,and inhibite postharvest decay at early storage.