猴头菌液体静置发酵液的化学成分与细胞毒活性

Chemical Components from Surface Liquid Fermented Hericium erinaceus with Cytotoxity Investigations

以马铃薯葡萄糖为培养基进行猴头菌液体静置发酵。利用萃取、中压液相色谱(RP-18 gel)、凝胶柱层析(Sephadex LH-20)和硅胶柱层析(Silica gel)从其发酵液中分离并结构鉴定了6个化合物。经核磁共振波谱解析与质谱鉴定,确定化合物的结构为Erinacine P(1)、Orsellinaldehyde(2)、Herierin IV(3)、Erinapyrone A(4)、Erinapyrone B(5)和Erinapyrone C(6)。Erinacine P(1)为首次从猴头菌发酵液中分离获得的二萜糖苷化合物,MTT实验表明其具有较强的细胞毒活性。Orsellinaldehyde(2)为首次从猴头菌中分离到的苯甲醛衍生物。Herierin IV(3)、Erinapyrone A(4)、Erinapyrone B(5)和Erinapyrone C(6)为4个吡喃酮衍生物。

Hericium er/naceus was cultured by surface liquid fermentation with Potato-Dextose medium. The fermented solution was extracted and purified by repeated column chromatography （RP-18 ,Sephadex LH-20 and silica gel） to afford six chemical components. Based on the NMR and ESI MS data, the structures of the six components were elucidated as efinacine P （ 1 ）, orsellinaldehyde （2）, herierin IV （3）, erinapyrone A （4）, erinapyrone B （ 5 ） and erinapyrone C （6）. Erinacine P （1） was isolated from the fermented solution of H. erinaceus for the first time and it showed high bioac- tivity in the MTT assay against HeLa ceils. Orseilinaldehyde （2）, named 2,4-dihydroxy-6-methylbenzaldehyde, was sep- arated from H. erinaceus for the first time. Herierin IV （3）, erinapyrone A （4）, erinapyrone B （5） and erinapyrone C （6） were all the pyrone derivatives.