彝族虫类药日本琵琶甲的化学成分研究

Chemical Constituents from the Insect Blaps japonensis

采用多种分离材料包括硅胶、Sephadex LH-20和RP-18从日本琵琶甲的50%乙醇提取物的正丁醇部位分离得到10个化合物,经过波谱学方法分别鉴定为Bracteanolide A(1)、3,4-Dihydroxyphenylacetic acid methyl ester(2)、Hydroxytyrosol(3)、3,4-Dihydroxy-β-methoxyphenethyl alcohol(4)、(S)-(+)-2-(3,4-Dihydroxy phenyl)-2-ethoxyl-ethanol(5)、3-Hydroxy-4-methoxy-phenylmethylcarbinol(6)、4-Hydroxybenzoic acid(7)、原儿茶醛(8)、Ethylβ-D-xylobioside(9)和Butylβ-D-xylobioside(10)。所有化合物均系从该种中首次分离。其中化合物1具有抑制脂多糖诱导的巨噬细胞一氧化氮产生的作用,可能为该昆虫消炎、消包块的物质基础之一。

Ten compounds were isolated from the ethanol extract of Blaps japonensis Marsetd. by the combined separation of silica gel, Sephadex LH-20 and RP-18 column chromatography. They were identified as Bracteanolide A （1）, 3,4-Di- hydroxyphenylacetic acid methyl ester （2）, Hydroxytyrosol （ 3 ）, 3,4-dihydroxy-13-methoxyplienetliyl alcohol （ 4 ）, （ S ） - （ ＋ ） -2- （3,4-Dihydroxy phenyl ） -2-ethoxyl-ethanol （ 5 ）, 3-Hydroxy-4-methoxy-phenylrnethylcarbinol （ 6 ）, 4-Hydroxy- benzoic acid （7）, Protocatechualdehyde （8）, Ethyl β-D-xylohioside （9） and Butyl β-D-xylobioside （ 10）, respectively, on the basis of NMR data and comparison with literatures. All the compounds were isolated from this species for the first time. and compound 1 could inhibit the production of nitric oxide of LPS-induced macrophage cells.