真菌竹黄双向调节TNF-α诱导的L929细胞凋亡过程

Fungus Shiraia Bambusicola Bi-directionally Regulating Apoptosis Induced by TNF-α in L929 Cell Line

真菌竹黄是我国特有的菌类药物,应用前景广阔,具有抗肿瘤和抗炎功效,但作用机理仍不十分清楚。我们以TNF-α诱导小鼠成纤维细胞(L929细胞株)为模型,深入探讨竹黄发挥药效的作用机理。研究发现,竹黄可以双向调节TNF-α诱导的L929细胞凋亡过程,高浓度竹黄可以促TNF-α诱导的细胞凋亡,低浓度竹黄具有抗TNF-α诱导的细胞凋亡作用。在竹黄抗凋亡过程中,信号网络中所涉及的关键蛋白COX-2表达下降,BCL-2表达上升,而NF-κB基本无明显变化。我们推测竹黄中含有药理作用相反的物质,通过调节信号网络中关键蛋白COX-2和BCL-2等的变化,进而改变信号网络平衡,发挥抗肿瘤与抗炎功效。相关研究有利于深入理解竹黄抗肿瘤与抗炎的药效作用,为运用生物活性导向和天然药物提取分离技术,进行相关新药的研发提供理论依据。

Fungus Shiraia bambusicola is endemic to China, having a bright future with anti-tumor and anti-inflammatory effect,but the mechanism of pharmacology is still not very clear. Using TNF-α-indueed mouse fibroblast cells （L929 cells） as a model,we further investigated Shiraia bambusicola＇ s pharmacological effects. The data showed that it could bi-direetionally regulate TNF-α-indueed apoptosis in L929 cells and many key proteins in the signal network play impor- tant roles in the process. During the anti-apoptosis process, western blot results indicated that COX-2 expression de- ereased,BCL-2 expression increased, while NF-KB expression had no significant change. We speculated that Shiraia bambusicola contained two kinds of materials with contrast pharmacological effects, which could change the balance of signal networks, and therefore showed an anti-tumor and anti-inflammatory effect. The research is helpful for further un- derstanding the fungus＇ anti-inflammatory and anti-tumor effect. Our results will provide a theoretical basis for the asso- ciated development of new drugs by the technology of biological activity-oriented, natural medicine extraction and separa- tion.