鄱阳湖野生河豚鱼体内河豚毒素产生菌的分离鉴定(英文)

Isolation and Identification of a Tetrodotoxin-Producing Bacterium from the Wild Puffer Fish of Poyang Lake

从中国鄱阳湖捕获的暗纹东方鲀卵巢中分离到4株菌,通过小鼠实验、薄层色谱、质谱分析及荧光分光光度法确认TL-1菌株发酵液中含河豚毒素。24℃培养5 d,通过活性炭柱、凝胶柱从TL-1发酵液中分离河豚毒素粗毒液。经形态、生理生化及16S rRNA分析鉴定,表明该菌属于花域芽孢杆菌属。本研究首次报道从鄱阳湖河豚鱼中分离到产毒菌。

Four bacterial strains were isolated from the ovary of Takifugu obscurus collected from Poyang lake of China. TL-1 was found can produce tetrodotoxin demonstrated by Mouse bioassay,thin-layer ehromatography,fluorimetric spec- trophotometry and eleetrospray ionization mass speetrometry. After being cultured at 24 ℃ for 5 days, the toxin was ex- tracted from the fermentation liquid of strain TL-1 by active charcoal column, gel-filtration chromatography column. Based on morphological, physiological, biochemical characteristics and 16S rRNA analysis, this strain was identified as Bacillus vallismortis. Our research was the first report about the tetrodotoxin- producing bacteria isolated from the wild puffer fish collected from Poyang lake.