发酵辅助提取对三七总皂苷提取物生物活性的影响

Effect of Fermentation-Assisted Extraction on the Bioactivity of Panax notoginseng Saponins

以DPPH自由基清除法、FRAP及Fe3+还原力法测定抗氧化能力,抑菌圈及最低抑菌浓度评价抑菌活性,对比发酵法与常规提取法所得三七总皂苷提取物活性差异,探讨微生物发酵提取三七总皂苷及其组分含量变化对生物活性的影响。结果显示,相比常规提取,发酵提取所得三七总皂苷含量提高35.36%,稀有皂苷组分Rh1、F1、Rg3含量增多,活性测试显示其DPPH清除的IC50由1.226降至0.377 mg/mL,对Fe3+还原力提高,对金黄色葡萄球菌、青霉菌等的抑制活性增强。结果说明发酵辅助提取能提高三七总皂苷的有效溶出,实现成分间转化,同时还能提高提取物生物学活性,具有重要的实际应用价值。

In this paper,the antioxidant and antimicrobial activities of Panax notoginseng Saponins （PNS） extracted by fermentation-assisted and traditional methods were evaluated and compared to elucidate the effects of microbial fermenta- tion on PNS contents and bioactivities. DPPH, FRAP and Fe3 ＋ reduction capability assays were employed to test antioxi- dant activity, inhibition zone and minimum inhibitory concentration were used to determine antimicrobiol activity. The re- suhs indicated that the total saponins contents of PNS by fermentation-assisted were increased by 35.36% comparing with traditional method, and the contents of rare ginsenosides Rh1, F1 , Rg3 were also increased. Due to fermentation, the IC50value of DPPH radical scavenging capacity was reduced from 1. 226 to 0.337 mg/mL. However, the Fe3 ＋ reduction capability and inhibitory activities to bacteria and fungi such as Staphylococcus aureus, PeniciUium sp. etc were en- hanced. This study demonstrated that fermentation-assisted extraction can increase the dissolution of active constituents from the materials,insert biotransformation into the system, and improve their bioactivities. The novel method provided useful information for future application in natural products extraction.