摘要
目的:建立HPLC法测定复方氨基酸注射液(18AA-Ⅳ)中5-羟甲基糠醛的含量。方法:采用Phenomenex PRODIGY ODS(3)C18100A分析柱(250 mm×4.6 mm,5μm),以磷酸盐缓冲液-甲醇(95∶5)为流动相,流速1.0ml/min,检测波长284 nm,柱温40℃。结果:本方法的线性范围为0.029 9~23.912μg/ml(r=0.999 6),平均回收率为99.10%,RSD为1.08%(n=9)。结论:本方法简便,专属性强,结果准确可靠,适用于复方氨基酸注射液(18AA-Ⅳ)中5-羟甲基糠醛的含量测定。
Objective : A HPLC method was developed to the determination of 5 - hydroxymethylfurfural in compound amino acid injection ( 18AA - Ⅳ). Methods: Phenomenex PRODIGY ODS(3 ) 100A column(250 mm ×4.6 mm,5 μm) was used. The mo- bile phase was phosphate buffer - methanol (95: 5 ). The flow rate was 1.0 ml/min,and the detection wavelength was 284 nm. Re- suits : The method was proved linear in the range of 0.029 89 - 23. 912 μg/ml ( r = 0.999 3 ). The average recovery of the method was 99.10% and RSD 1.08% (n = 9 ). Conclusion: This method is proved to be simple and rapid, accurate and issuitable for de- termination of 5 - hydroxymethylfuffural in compound amino acid injection ( 18AA - Ⅳ ) .
出处
《天津药学》
2012年第6期4-6,共3页
Tianjin Pharmacy