氨态氮、亚硝态氮、硝态氮对麦穗鱼的急性毒性研究

Studies on Acute Toxicity of Ammoniacal Nitrogen,Nitrite,and Nitrate for Pseudorasbora parva

为了对麦穗鱼的人工养殖,以及作为水生毒理学的实验材料提供参考。在水温(12.7±1)℃、溶氧5.60～9.87mg/L的条件下,研究了氨态氮、亚硝态氮、硝态氮对麦穗鱼的急性毒性。试验结果表明:非离子氨对麦穗鱼的24、48、72、96h的LC50值(95%置信区间)分别为0.435mg/L(0.407～0.466mg/L)、0.384mg/L(0.356～0.417mg/L)、0.371mg/L(0.344～0.402mg/L)、0.346mg/L(0.316～0.383mg/L),非离子氨对麦穗鱼的安全浓度是0.0346mg/L。亚硝态氮对麦穗鱼的24、48、72、96h的LC50值(95%置信区间)分别为422.862mg/L(365.741～490.035mg/L)、327.746mg/L(276.875～394.054mg/L)、266.291mg/L(228.302～310.877mg/L)、236.907mg/L(200.488～281.108mg/L),亚硝态氮对麦穗鱼的安全浓度是23.6907mg/L。硝态氮对麦穗鱼的24、48、72、96h的LC50值(95%置信区间)分别为2.155g/L(2.074～2.242g/L)、1.929g/L(1.864～1.999g/L)、1.769g/L(1.701～1.842g/L)、1.663g/L(1.577～1.780g/L),硝态氮对麦穗鱼的安全浓度是0.1663g/L。在3种化合物中,非离子氨对麦穗鱼毒性最大,亚硝态氮次之,硝态氮对麦穗鱼的毒性最小。

In order to provide reference for pseudorasbora parva artificial culture and as aquatic toxicology experimental material. The acute toxic effects of ammoniacal nitrogen, nitrite, and nitrate on pseudorasbora- parva were studied at temperature （12.7±）℃ and dissolved oxygen 5.60-9.87 mg/L. The test results showed that, the 24 h-LC50, 48 h-LC50, 72 h-LC50 and 96 h-LC50 （95 % confidence interval） of non-ionic ammonia to P. parva were 0.435 mg/L （0.407-0.466 rag/L）, 0.384 mg/L （0.35-0.417 mg/L）, 0.371 mg/L （0.344- 0.402 mg/L）, 0.346 mg/L （0.316-0.383 mg/L）, respectively. The safety concentration of non-ionic ammonia to P. parva was 0.0346 mg/L. The 24 h-LC50, 48 h-LC50, 72 h-LC50 and 96 h-LC50 （95 % confidence interval） of nitrite to P. parva were 422.862 mg/L （365.74-490.035 mg/L）, 327.746 mg/L （276.875-394.054 mg/L）, 266.291 mg/L （228.302-310.877 mg/L）, 236.907 mg/L （200.488-281.108 mg/L）, separately. The safety concentration of nitrite to P. parva was 23.6907 mg/L. The 24 h-LC50, 48h-LC50, 72 h-LC50 and 96 h-LC50 （95 % confidence interval） of nitrate to P. parva were 2.155 g/L （2.074-2.242 g/L）, 1.929 g/L （1.864-1.999 g/L）, 1.769 g/L （1.701-1.842 g/L）, 1.663 g/L （1.577-1.780 g/L）, respectively. The safety concentration of nitrate to P. parva was 0.1663 g/L. In three kinds of compound, the toxicity of non-ionic ammonia to P. parva was maximum, nitrite was the second smallest, and the toxicity of nitrate to P. parva was minimum.