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D-半乳糖-1-^(13)C的合成与表征 被引量：1

Synthesis and Characterization of D-Galactose-1-^(13)C
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摘要 以硝基甲烷-13 C为原料合成D-半乳糖-1-13 C。采用分离中间体硝基糖醇的方式,简便拆分差向异构体D-半乳糖和D-塔罗糖,合成产物的总产率为42.6%,13 C丰度为99.1%,光学纯度为100%,化学纯度为99.7%。以高效液相色谱-蒸发散射光检测器(HPLC-ELSD)、液相色谱-质谱联用仪(LC-MS)和核磁共振(1 H NMR)对所得产物进行结构表征,确定产物结构为D-半乳糖-1-13 C。 D-Galactose-1-13C was synthesized with nitromethane-13C as starting material.The separation was performed of the epimers of nitro-D-Galactitol and nitro-D-talitol,which resulted in D-Galactose being obtained and the resolution of the epimers of D-Galactose and D-taltose being avoidance.D-Galactose-1-13C was characterized by HPLC-ELSD,LC-MS and 1H NMR.The fine structure and 13C abundance of it were done by LC-MS and 1H NMR.The yield of 13C-labeled D-Galactose was 42.6% with 99.1% 13C abundance,100.0% optical purity and 99.7% chemical purity.
作者 卢伟京 吴开成 李帅 徐仲杰
机构地区 上海化工研究院
出处 《同位素》 CAS 2012年第4期219-227,共9页 Journal of Isotopes
基金 国家高技术研究发展计划项目(2008AA02Z427)
关键词 D-半乳糖-1-13C 合成 13C标记 D-Galactose-1-13C synthesis 13C-labeled
分类号 O612.4 [理学—无机化学]
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参考文献24

  • 1Protocols 13C Breath Tests - LIVER[M]. Campro Scientific GmbH (Germany).
  • 2Perri F, Marras RML, Ricciardi R, et al. 13 C- Breath Tests in hepatology (cytosolic liver func- tion) [J].Eur Rev Med Pharmacol Sci, 2004, 8 (1) : 47-49.
  • 3Saadeb S, Behrens PW, Parsi MA , et al. The u tility of the la C-galactose breath test as a measure of liver function [J]. Aliment Pharmacol Ther, 2003, 18(10): 995-1 002.
  • 4Mion F, Rousseau M, Scoazec JY, et al. [13C] Galactose breath test: correlation with liver fibro sis in chronic hepatitis C[J].Eur J Clin Invest 1999, 29(7): 624-629.
  • 5Giannini EG, Fasoli AA, Borro P, et al. 13C-ga- lactose breath test and 13 C-aminopyrine breath test for the study of liver function in chronic liver dis- ease[J]. Clin Gastroenterol Hepatol, 2005, 3 (3) : 279 285.
  • 6Holtmeier J, Leuschner M, Schneider A, et al. 13C-methacetin and 13C-galactose breath tests can assess restricted liver function even in early stages of primary biliary cirrhosis[J]. Scand J Gastroen- terol, 2006, 41(11): 1 336-1 341.
  • 7Suzuki S, Ishii Y, Asai S, et al. [l-13C]Galactose breath test For quantitative measurement of liverfunction in a short period[J]. Digestion, 2000, 62 (2-3) : 194-199.
  • 8Suzuki S, Ishii Y, Asai S, et al. [1-13C] Breath Test of Galactose and Fructose for Quantitative Liver Function[J].Surg Res, 2001, 96 (1): 90-95.
  • 9Giannini EG, Testa R. 13 C-Breath Tests and liver fibrosis[J].Eur Rev Med Pharmacol Sci, 2004, 8 (1) : 51-54.
  • 10Sato K, Akai S, Sakuma M. Practical synthesis of [1-13C]- and [6-13C]-D-galactose[J]. Tetrahed- ton Lett, 2003, 44(26): 4 903-4 907.

二级参考文献12

  • 1Lane EA.The aminopyrine breath test for the evaluation of liver function in alcoholic patients:drug pharmacokinetics and environmental factors[J].Adv Alcohol Subst Abuse,1988,7(3-4):25-32.
  • 2Krumbiengel P,Günther K,Faust H,et al.Nuclear medicine liver function tests for pregnant women and children:1.Breath tests with 14C-methacetin and 13C-methacetin[J].Eur J Nucl Med,1985,10(3-4):129-133.
  • 3Klatt S,Taut C,Mayer D,et al.Evaluation of the 13C-methacetin breath test for quantitative liver function testing[J].Z Gastroenterol,1997,35(8):609-614.
  • 4Baruque SL,Razquin M,Jimenez I,et al.13C-phenylalanine and 13C-methacetin breath test to evaluate functional capacity of hepatocyte in chronic liver disease[J].Digest Liver Dis,2000,32(3):226-232.
  • 5Zipprich A,Meiss F,Steudel N,et al.13C-Meth-acetin metabolism in patients with cirrhosis:relation to disease severity,haemoglobin content and oxygen supply[J].Aliment Pharmacol Ther,2003,17(12):1 559-1 562.
  • 6Petrolati A,Festi D,De Berardinis G,et al.13C-Methacetin breath test for monitoring hepatic function in cirrhotic patients before and after liver transplantation[J].Aliment Pharmacol Ther,2003,18(8):785-790.
  • 7Schneider JF,Scholler DA,Schreider BD.Use of 13C-phenacetin and 13C-methacetin for the detection of alterations in hepatic drug metabolism[C]//Klein ER,Klein PD,eds.Stable isotopes:proceedings of the third international conference.New York:Academic Press,1979:507-516.
  • 8Herrmann M,Krumbiegel P,Moebius G.Synth-ese und massenspektrometrische prüfung der stabilisotop markierten arzneimittel[15N]Methacetin and[13C,15N]Methacetin[J].Isotopenpraxis,1986,22(5):185-187.
  • 9Shirin H,Aeed H,Shalev T,et al.Utility of a 13C-methacetin breath test in evaluating hepatic injury in rats[J].J Gastroen Hepatol,2008,23(11):1 762-1 768.
  • 10Matsumoto K,Suehiro M,Iio M,et al.[13C]methacetin breath test for evaluation of liver damage[J].Dig Dis Sci,1987,32(4):344-348.

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  • 5GYGI S P, RIST B, GERBER S A, et al. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags [ J ]. Nat. Biotechnol. , 1999,17 ( 10 ) : 994- 999.
  • 6ONG S E, BLAGOEV B, KRATCHMAROVAL I, et al. Stable isotope labeling by amino acids in cell culture, SI- LAC, as a simple and accurate approach to expression proteomics [ J ]. Mol. Cell Proteomics. , 2002,1 ( 5 ) : 376- 386.
  • 7VANDER, ZANDEN M J, FETZER W W. Global pat- terns of aquatic food chain length [J]. Oikos,2007,116 (8) :1 378-1 388.
  • 8GORANKA C,WOLFGANG S. Determination of dithio- carbamate fungicide residues by liquid chromatography/ mass spectrometry and stable isotope dilution assay[J]. Rapid Commun. Mass Spectrom. , 2007,21 ( 24 ) : 4 009- 4 016.
  • 9LISSALDE S, MAZZELLA N, FAUVELLE V, et al. Liq- uid chromatography coupled with tandem mass spectrom- etry method for thirty-three pesticides in natural water and comparison of performance between classical solid phase extraction and passive sampling approaches [J]. J. Chromatogr. A,1 218(2 011) :1 492-1 502.
  • 10MULDER P P J,BEUMER B,VAN RHIJN J A. The de- termination of biurea : a novel method to discriminate be- tween nitrofurazone and azodicarbonamide use in food products[J]. Anal. Chim. Acta, 2007,586 ( 1/2 ) : 366- 373.

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2012年 第4期

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