摘要
用不同方法进行普通烟草叶肉细胞原生质体培养 ,在低温 ( 1 0℃ )预处理原生质体分离材料后 ,加厚双层培养基中的固相至固液相体积比为 4∶ 1～ 5∶ 1时 。
Different methods are used to cultivate leaf protoplast of Nicotiana tabacum .After pre treatment of protoplast material at low temperature (10℃),two later culture medium is thickened to the extent that the volume redio of solid and liquid reached 4∶1-5∶1,which is quite helpful for rapid emergence of callus and plant regeneration.
出处
《西北农业大学学报》
CSCD
北大核心
2000年第3期101-103,共3页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
基金
安徽省科委"八五"攻关项目!"主要农作物细胞育种及遗传操作研究"
