青天葵走茎诱导与增殖的初步探究

Preliminary study for Nervilia fordii amphitropous stems Induction and proliferation

目的:初步探究适合青天葵走茎诱导与增殖的激素配比和方法,为青天葵大规模组织培养提供原材料和工厂化育苗提供技术支撑.方法:以青天葵叶片、叶柄、球茎、根为外植体进行走茎诱导,同时探究不同激素配比对走茎增殖及走茎诱导球茎的影响.结果:在走茎诱导中以球茎为原材料诱导效果较好,诱导率为83.33%,以MS+6-BA1.0 mg/L+IBA2.0 mg/L和MS+6-BA1.0 mg/L+2,4-D1.0 mg/L作为基本培养诱导效果较佳;在走茎的继代增殖中以MS+6-BA 1.0mg/L+2,4-D0.5mg/L的效果较好,增殖倍数可达到20.47;在MS+0.2 mg/L6-BA的基本培养基中加入2,4-D1.0mg/L和IBA0.5mg/L能使走茎诱导产生球茎,并且在加入2,4-D1.0mg/L的培养基中诱导产生的球茎数量及直径较明显.

Objective： in order to provide raw materials and technical support in large-scale tissue culture and Industrialization of Seedling Production, amphitropous stem of Nervilia fordii was induced and proliferated by different media and hormone Proportions. Methods： leaves, petioles, corms and roots of Nervilia fordii as explant to study the camphitropous stems proliferation and induced to petiole under different media and homlone Proportions. Results： the maximum rate of arnphitropous stem induction was 88.33% and occurred on the MS medium supplemented with 1.0 mg/L 6-BA and 2.0 mg/L IBA and MS medium supplemented with 1.0 mg/L 6-BA and 1.0 mg/L 2,4-D when using corms as explant. The maximum rate of amphitropous stem proliferation was 20.47 and occurred on the MS medium supplemented with 1.0 mg/L 6-BA and 0.5 mg/L 2,4-1）. Amphitropous stem was induced to corm in MS medium supplemented with 0. 2 mg/L 6- BA, 1.0mg/L 2,4-D and 0.5mg/L IBA but the best corm cultivation medium was MS medium supplemented with 0. 2 mg/L 6 - BA and 1.0mg/L 2,4-D.