Notch1表达与乳腺癌细胞迁移及侵袭能力的相关性

Relationship between Expression of Notch1 and Ability of Migration and Invasion in Breast Cancer Cells

目的研究Notch1的表达与乳腺癌细胞迁移侵袭能力的相关性。方法以RT-qPCR及Western blot检测细胞株MCF-7及MCF-7/ADR中Notch1基因及蛋白的表达情况;以Transwell小室进行迁移实验和侵袭实验检测上述细胞株的迁移、侵袭能力。结果 MCF-7/ADR的Notch1mRNA表达水平明显高于MCF-7[(0.074 8±0.004 3)vs.(0.046 1±0.002 2)],差异有统计学意义(P<0.05);MCF-7/ADR的Notch1蛋白表达水平明显高于MCF-7[(1.636 0±0.042 2)vs.(0.622 0±0.028 6)],差异有统计学意义(P<0.05);MCF-7/ADR的迁移及侵袭的穿膜细胞数均明显多于MCF-7的穿膜细胞数[(60.16±5.27)vs.(13.88±3.16);(13.62±2.25)vs.(9.62±1.30)],差异均有统计学意义(均P<0.05)。Notch1mRNA及蛋白的表达水平与乳腺癌细胞侵袭、迁移能力呈正相关。结论 Notch1信号通路参与调控乳腺癌细胞的侵袭与迁移能力,阻断该通路活化将可能抑制乳腺癌细胞的侵袭、迁移。

Objective To investigate the relationship between the expression of Notch1 and the ability of migration and invasion in breast cancer cells.Methods RT-qPCR and Western blot were employed to detect the expression status of Notch1 in breast cancer cell lines MCF-7 and MCF-7/ADR.Transwell chambers were used to detect the difference in migration and invasion ability between MCF-7 and MCF-7/ADR.Results The mRNA and protein expression levels of Notch1 were both significantly higher in MCF-7/ADR than in MCF-7 [（0.074 8±0.004 3）vs.（0.046 1±0.002 2）,and（1.636 0±0.042 2）vs.（0.622 0±0.028 6）,P0.05].There was significant difference in the number of migatory and invasive cells between MCF-7/ADR and MCF-7 [（60.16±5.27）vs.（13.88±3.16）,P0.05;（13.62±2.25）vs.（9.62±1.30）,P0.05].The correlation between the expression of Notch1 and the ability of migration and invasion in breast cancer cells was significant.Conclusion The inhibition of Notch1 signaling pathway may block the process of invasion and migration of breast cancer cells,suggesting Notch1 signaling pathway may be involved in regulation of invasion and migration.