中草药抗热应激蛋鸡小肠上皮内淋巴细胞的组织学观察

Influence of Chinese herbal medicine on intraepithelial lymphocyte under heat stress in layers

本试验旨在研究蛋鸡热应激情况下中草药对其小肠上皮内淋巴细胞的影响。选取120只88日龄蛋鸡,随机平均分为6个组。任取其中3个组为中草药试验组,分别加入高、中、低浓度的中草药;另3个组为对照组,一组加入维生素C,一组置于高温环境中,一组于室温条件下饲养。分别在试验的第1天、第4天、第8天和第10天随机选取5只鸡致死,取小肠部分做石蜡切片,H.E染色后在显微镜下观察小肠上皮内淋巴细胞的变化。试验表明,添加中草药的试验组小肠上皮内淋巴细胞数显著多于对照组,且浓度高的效果好。由此显示了中草药可有效对抗热应激,提高蛋鸡生理机能。

This experiment was designed to study the influence of Chinese herbal medicine on intraepithelial lymphocyte under heat stress in layers. A total of 120 88-day-old layers were randomly assigned to 6 groups, in which 3 groups were treated as experimental groups and the others were treated as the control. The experimen- tal birds were fed basal diet added with high, medium and low levels of Chinese herbal medicine under heat stress, respectively. The control birds were fed basal diet under room or high temperature, or basal diet added with vitamin C under heat stress,respectively. Then five layers of each group were randomly selected and killed on the first, fourth, eighth and tenth day. The small intestine was taken and made sections, and then observed the changes of intraepithelial lymphocyte under the microscope after H. E staining. The results showed that lay- ers fed diet added with Chinese herbal medicine had more intraepithelial lymphocytes than the control birds,and the intraepithelial lymphocytes increased as the added level increased. All of these showed that Chinese herbal medicine can be effective against heat stress and improve the physiological function of layers.