空间环境诱导褪色沙雷菌LCT-SM166的蛋白质组学分析

Proteomics of space serratia marcescens LCT-SM166 strain

目的分析和鉴定神舟八号飞船搭载褪色沙雷菌的差异表达蛋白质。方法对神州八号飞船搭载的褪色沙雷菌LCT-SM166及地面对照组LCT-SM213进行分离并进行16sRNA鉴定,采用同重同位素相对与绝对定量(isobaric tags for relativeand absolute quantitation,iTRAQ)方法对褪色沙雷菌LCT-SM166和LCT-SM213进行蛋白质组质谱检测,分析两株菌的差异表达蛋白。结果共鉴定到1 713个蛋白质,LCT-SM166与LCT-SM213的差异蛋白组学分析发现了111个蛋白质表达的改变,其中29个蛋白表达上调,91个蛋白表达下调。基因本体论(gene ontology,GO)功能富集分析显示大多数差异蛋白质主要与能量代谢有关。结论空间环境可影响褪色沙雷菌大量蛋白质的表达,差异表达蛋白主要分布在代谢相关过程。空间诱变菌株的蛋白质组学研究为后续贯穿组学的研究奠定基础。

Objective To analyze and identify the proteins with different expressions in serratia marcescens loaded in Shenzhou 8 spacecraft. Methods The serratia marcescens strain LCT-SM166 loaded in Shenzhou 8 spacecraft and ground control strain LCTSM213 were isolated and their 16sRN was identified by isobaric tags for relative and absolute quantitation （iTRAQ）. The proteins of serratia marcescens LCT-SM166 and LCT-SM213 strains were detected by mass spectrometry and their differential expression was analyzed. Results Among the 111 differentially expressed proteins in the 1 713 possible proteins identified in this study, the expression was up-regulated in 20 and down-regulated in 91. Gene ontology （GO） enrichment analysis showed that the majority of differentially expressed proteins were related to bacterial metabolism. Conclusion Space environment can affect the protein expressions of serratia marcescens. The differentially expressed proteins mainly participate in the bacterial metabolic process. Proteomic analysis of space serratia marcescens mutants can lay a foundation for further transomics analysis.