氢氧化铝干粉与凝胶制作大鼠哮喘模型的效果比较

Efficiency of rat asthma model established with aluminum hydroxide powder and gel

目的比较利用佐剂氢氧化铝干粉与氢氧化铝凝胶制作大鼠过敏性哮喘模型的建模效果。方法 30只清洁级健康雄性Wistar大鼠随机分为A组(正常对照组)、B组(氢氧化铝凝胶造模组)和C组(氢氧化铝干粉造模组),每组10只。建模后通过观察大鼠行为学改变,检测各组大鼠支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中细胞变化、肺组织匀浆中细胞因子水平变化及肺组织病理学改变,并评价模型效果。结果 1)B组与C组均出现哮喘症状,但C组发病程度轻于B组。2)B组与C组BALF中细胞总数和嗜酸细胞(eosinophils,EOS)百分比均高于A组(P<0.05),且B组大鼠BALF中细胞总数和EOS百分比显著高于C组(P<0.05)。3)B组与C组肺组织匀浆中IFN-γ含量显著低于A组(P<0.01)。4)B组与C组都表现出哮喘炎症反应的病理改变,其中以B组更为显著。结论氢氧化铝凝胶增强由卵清蛋白(ovalbumin,OVA)所致的过敏反应模型效果优于氢氧化铝干粉模型。

Objective To compare the efficiency of rat asthma model established with aluminum hydroxide powder and gel. Methods Thirt healthy male Wistar rats were randomly divided into group A（normal control group）, group B（aluminum hydroxide gel modeling group） and group C（aluminum hydroxide powder modeling group）. After the model was established, its efficiency was assessed by observing behaviors of rats, detecting cells in bronchoalveolar lavage fluid（BALF）, measuring cytokine levels in lung tissue homogenates and observing pulmonary pathological changes. Results Symptoms of asthma occurred in groups B and C, but they were severer in group B than in group C. The total number of cells and the percentage of eosinophils（EOS） in BALF were significantly higher in groups B and C than in group A（P 〈 0.05） and in group B than in group C（P 〈 0.05）. The IFN- γ levels in lung tissue homogenates were significantly lower in groups B and C than in group A（P 〈 0.01）. The pathological changes induced by inflammatory reaction occurred in groups B and C and were more significant in group B than in group C. Conclusion The efficiency of aluminum hydroxide gel is better than that of aluminum hydroxide powder in increasing ovalbumin-induced allergic response in animal asthma model.