用PCR检测鸡大肠杆菌耐热肠毒素基因

Detection of ST Gene of Avian Escherichia coli by Polymerase Chain Reaction(PCR)

用１对合成的特异性核苷酸片段，通过聚合酶链反应（ＰＣＲ），以鸡致病性大肠菌２０个血清型菌株提取的染色体为模板进行扩增，标准阳性对照（ｐＳＬＭ４００）及扩增到 １６个大小为 １５０ ｈｐ的 ＤＮＡ阳性片段，其它 ６株及阳性对照株 ｐＥＷＤ２９９未扩增到产物。斑点杂交表明，所扩增的阳性条带与标准耐热肠毒素基因（ ＳＴ）杂交后呈强阳性。

Based on the Published ST gene nucleotide sequences from human Escherichia coli, a pair Of Primers were desighed and synthesized, 16 positive 150 bp products were generated by polymerase chain reaction(PCR) from 20 chromosomal samples of different stereotype Escherichia coli strains. Hybridization results showed that these 150 bp positive products were identical to the reference E. coli ST gene as detected by dot-blot hybridization.