反复胚胎种植失败患者HLA-Cw基因多态性的研究

Study on polymorphism of HLA-Cw in patients with repeated implantation failure

目的初探HLA-Cw基因多态性与反复胚胎种植失败的关系。方法采用聚合酶链反应-直接碱基序列分析基因分型技术的方法对研究组50例反复胚胎种植失败患者和对照组17例一次体外受精-胚胎移植即获得妊娠并分娩活婴的患者进行HLA-Cw基因多态性检测,并采用SPSS17.0软件进行分析。结果 (1)在HLA-Cw*01、HLA-Cw*03、HLA-Cw*07基因位点中,研究组和对照组均有较高的频率;(2)研究组HLA-Cw*06基因频率较对照组高,HLA-Cw*07基因频率低于对照组;(3)研究组和对照组的HLA-Cw^(asn)频率远高于HLA-Cw^(lys);(4)研究组的HLA-Cw^(asn)频率略低于对照组,HLA-Cw^(lys)频率略高于对照组;(5)研究组夫妇双方均未检出HLA-Cw^(lys)等位基因的比例较对照组低,而至少一方检出至少一个HLA-Cw^(lys)等位基因的比例则较高。上述各观察指标两组间均无显著性差异(P>0.05)。结论 HLA-Cw^(lys)频率在反复胚胎种植失败患者中有增加趋势,虽无显著性差异,但有待扩大样本量进行进一步统计分析。

Objective： To investigate the correlation between the HLA-Cw gene polymorphism and the repeated implantation failure. Methods： Fifty patients with repeated implantation failure（study group）were matched to 17 patients who got pregnancy and had living birth from the first in vitro fertilization embryo transfer cycle（control group）. The genotyping of HLA-Cw gene detected by a polymerase chain reaction-sequence based genotyping（PCR-SBT）method. The data of the two groups was analyzed by the SPSS17.0 software. Results： The results of both groups showed a high gene frequency in HLA-Cw ~ 01, HLA-Cw ~ 03 and HLA-Cw ~ 07. The gene frequency of HLA-Cw ~ 06 in the study group washigher than that in the control group. The gene frequency of HLA-Cw ~ 07 in the study group was lower than that in the control group. In both groups,their HLA-Cw~ frequency was much higher than HLA-Cw^y~ frequency. The study group had less HLA-Cwa^n gene frequency but more HLA-Cwly~ gene frequency than the control group. Compared with the control group,the percentage of couples whose HLA-Cwly~ allele could not be detected was lower, but the percentage of one or both of spouses whose one or more the HLA-Cwlys allele could be detected in the study group was higher. All of those observation indexes above showed no significant difference between two groups（P〉0.05）. Conclusions： The gene frequency of HLA-Cwlys shows an increased tendency in the repeated implantation failure patients. Although there is no significant difference, it is expected to enlarge the sample size for a further statistic analysis.