子痫前期患者血清中抗血管紧张素Ⅱ1型受体抗体对大鼠小动脉功能的影响

Effect of autoantibody of angiotensinⅡtype 1 receptor in serum of preeclampsia patients on small vascular function in rat

目的采用微血管环技术,观察抗血管紧张素II 1型受体的自身抗体(ATl-AA)对主要脏器的小血管(包括冠状动脉、大脑中动脉、肾动脉、子宫动脉和肠系膜动脉)的直接作用,明确该自身抗体对子痫前期患者主要脏器的影响。方法利用MAb Trap Kit试剂盒对子痫前期患者抗体阳性者血清中的抗体IgG进行提取和纯化,用血管环技术检测纯化后的抗体对大鼠小动脉收缩功能的影响。结果 AT1-AA浓度依赖性收缩大脑中动脉和冠状动脉,1.0μmol/L的AT1-AA可使大鼠大脑中动脉和冠状动脉的最大收缩张力从对照组的(0.03±0.02)mN分别增加到(0.82±0.09)mN和(2.51±0.19)mN,而对肾动脉、肠系膜动脉和子宫动脉的收缩作用不明显。结论 AT1-AA浓度依赖性地增强大鼠大脑中动脉和冠状动脉的收缩,可能是引起妊娠高血压综合征的原因之一。

Objective： To study the effect of autoantibody against angiotensin II type 1 receptor（AT 1-AA）in serum of preeclampsia patients on rat small vascular function（middle cerebral artery, coronary artery, mesenteric, renal and uterine arteries）, and investigate the role of AT 1-AA in pathogenesis of preeclampsia. Methods. Vasoconstrictive effect of purified IgG from the sera of either preeclampsia patients or normal pregnant women on isolated small arteries of rat was determined. Results. The results showed that AT1-AA showed variant effects on small vascular rings isolated from different organs. Of these vessels, middle cerebral artery and coronary artery were remarkably contracted by AT1-AA. A dose of 1.0 ~mol/L AT1-AA increased vasoconstrictive tensile force of aortic middle cerebral artery and coronary artery vascular rings from （0.03 0.02） mN to （0. 82 0. 09） mN and （2.51 ＋ 0.19）mN respectively. The changes of tensile force of mesenteric, renal and uterine arteries were not significant. Conclusions： These findings suggest that AT1-AA is a novel risk factor in pregnant women and might play a causative role in the development of preeclampsia.