复方乳酸钠葡萄糖注射液乳酸钠含量测定

Determination of sodium lactate in compound sodium lactate glucose injection

目的建立吸收度比值法测定复方乳酸钠葡萄糖注射液中乳酸钠的含量。方法采用紫外可见分光光度法在435nm与544nm波长处测定吸收度(A1、A2),求出吸收度比值(rr=A2/A1)。结果利用r值对x值进行线性回归,回归方程x=1.353-1.812r,相关系数为0.995,方法的平均回收率为100.5%,RSD为0.45%,与药典方法比较,测定4批样品,误差为±0.4%,结果无明显差异。结论该方法操作简便、快速、准确,可以用吸收度比值法代替离子交换树脂法测定乳酸钠含量。

Objective To establish absorbance ratio method for the determination of assay of sodium lactate in compound sodium lactate and glucose injection： Methods The ultraviolet visible spectrophotometry in determination of 435 nm and 544 nm wavelength absorbance （A1, A2）, calculated the absorption ratio r（r= A2/A1 ）. Results Using r on the x values of linear regression, the regression equation was x=l.353-1.812r, the correlation coefficient was 0.995, the average recovery was 100.5%. RSD was 0.45%.Compared with the pharmacopoeia method, determination of 4 batches of sample, the error was ±0.4%.There was no difference in outcome. Conclusion The method is simple, rapid, accurate, and can use the absorbance ratio method instead of ion exchange resin method for the determination of the content of sodium lactate.