亚砷酸钠对黑色素瘤细胞A375和G361色素代谢的影响

Effects of sodium arsenite on melanin metabolism of A375 and G361 cell lines

目的探讨亚砷酸钠（NaAs02）暴露对黑色素瘤细胞A375（以下简称A375）和G361（以下简称G361）的色素水平及酪氨酸酶（TYR）活力的影响，以及两种细胞色素代谢能力的差异。方法以0．0（对照）、0．1、1.0μmol／LNaAsO2作用于A375和G361细胞，72h后，以阿尔玛蓝还原法检测细胞活力水平，同时测定细胞中黑色素水平及TYR的活力水平。结果NaAs02作用72h后，0．1μmol／L剂量组的A375和G361细胞均呈轻度增殖表现，细胞活力[A375：（103．32±1．26）％；G361：（104．10±1．76）％]与对照组[A375：（100．00±1．08）％；G361：（100．00±1．79）％]比较，差异无统计学意义（P均〉0．05）；1．0p^mol／L剂量组细胞活力[A375：（75．32±1．59）％；G361：（78．26±2．10）％]与对照组相比均显著下降（P均〈0．05）。对照、0．1、1．0μmol／L剂量组的G361细胞的黑色素水平[（7．19±0．35）、（7．34±0．83）、（8．19±0．86）Pg／细胞]均高于A375细胞[（4．35±0．72）、（4．54±0．01）、（4．60±0．59）pg／细胞，P均〈0．05]，G361细胞的TYR活力水平[（54．13±1．21）、（54．56±0．21）、（56．25±0．85）贝克勒尔（Bq）]均高于A375细胞[（42．00±0．21）、（42．90±0．54）、（42．91±0．01）Bq，P均〈0．05]。2种细胞的黑色素生成水平及TYR活力水平均随NaAs02剂量增高而呈增高趋势，但组间比较，差异均无统计学意义（P均〉0．05）。结论砷致色素代谢异常可能与砷暴露致黑色素水平及’IYR活力增高有关；不同个体间色素代谢差异，可能与个体的黑色素代谢本底水平及TYR本底活力差异有关。

Objective To study the impact of sodium arsenite（NaAsO2） exposure on melanoma ceils A375 （hereinafter referred to as the A375） and G361 （hereinafter referred to as the G361） pigment production and tyrosinase （TYR） activity and the differences of pigment metabolism capacity between the cell lines. Methods A375 and G361 cells were exposed to sodium arsenite at concentrations of 0.0（control）, 0.1 and 1.0 μmol/L for 72 hours. Cell viability was measured by Alamar Blue assay. Melanin levels and TYR activity were measured at the same time. Results After exposure for 72 hours, the cells of 0.1 μmol/L dose groups of both of the two cell lines [A375：（103.32 ± 1.26）%; G361：（104.10 ± 1.76）%] showed a slight inerease of proliferation without significant differences compared with those of the control[A375：（100.00 ± 1.08）%; G361 ： （100.00 ± 1.79）%, all P 〈 0.05 ]; while cell viability of the 1.0 μmol/L dose group of both of the two ceil lines[A375：（75.32 ± 1.59）%; G361： （78.26 ± 2.10）%] were significandy lower than those of the control （all P 〈 0.05）. Melanin levels of G361 cell line [ （7.19 ＋ 0.35）, （7.34 ± 0.83）, （8.19 ± 0.86）pg/cell] were significantly higher than that of A375 [ （4.35 ± 0.72）, （4.54 ＋ 0.01）, （4.60 ± 0.59）pg/cell, all P 〈 0.05] in all the three groups. TYR activity of G361 cell line [ （54.13 ± 1.21）, （54.56 ± 0.21）, （56.25 ± 0.85）Bq] were also markedly higher than that of A375 cell[ （42.00 ± 0.21）, （42.90 ± 0.54）, （42.91 ± 0.01）Bq, all P 〈 0.05] in all the three groups. The melanin levels and TYR activities of both of the two ceils lines showed an increase tendency along with increased doses of arsenic exposure, but without significant differences when compared with those of the three groups（all P 〉 0.05）. Conclusions Arsenic related pigment disorder may be associated with increased melanin levels and TYR activities induced by arsenic exposure; individual difference of pigment metabolism may be associated with different basal melanin levels and TYR activity between different individuals.