酪氨酸对体外培养大鼠甲状腺细胞功能相关基因表达的影响

Effects of tyrosine on expression of thyroid cell function related gene in primary cultured rat cells

目的研究酪氨酸对体外培养大鼠甲状腺细胞甲状腺球蛋白（TG）、甲状腺过氧化物酶（TPO）、钠碘转运体（NIS）及促甲状腺素受体（TSHR）mRNA表达的影响。方法将体外培养大鼠甲状腺细胞分为对照组、酪氨酸I组、酪氨酸Ⅱ组，每组5例，分别用含0、5％、10％酪氨酸的培养液培养96h，收集甲状腺细胞，应用实时荧光定量PCR检测TG、TPO、NIS、TSHRmRNA表达。结果对照组、酪氨酸I组、酪氨酸Ⅱ组TG、NISmRNA表达分别为1．11±0．36、2．08±0．45、2．52±0．65和1．21±0．36、1．94±0．57、2．10±0．56，组间比较差异有统计学意义（F值分别为10．48、4．42，P均〈0．05），其中酪氨酸I组、酪氨酸Ⅱ组TG、NISmRNA表达明显高于对照组（P均〈0．05）。上述3组TPO、TSHRmRNA表达分别为1．02±0．54、1．14±0．29、1．17±0．38和0．81±0．44、0．91±0．30、0．90±0．19，组间比较差异无统计学意义（F值分别为0．18、0．26，P均〉0．05）。结论酪氨酸可能影响甲状腺细胞功能相关基因表达，增加碘在甲状腺的储存，通过作用于碘泵，调节甲状腺细胞的碘摄取功能。

Objective To investigate the effect of tyrosine on mRNA expression of thyroglobulin （TG）, thyroid peroxidase （TPO）, sodium/iodine symporter （NIS） and thyroid-stimulating hormone receptor （TSHR） in primary cultured rat thyroid cells. Methods Primary cultured rat thyroid cells were divided into three groups（cells from 5 rats in each group） ： control group, tyrosine group I, and tyrosine group 11 , and tyrosine of 0, 5%, 10% was added to the culture medium, and the cells were collected after cultured for 96 h, respectively. The mRNA levels of TG, TPO, NIS and TSHR of thyrocyte were determined by real-time quantitative PCR. Results The expression levels of TG, NIS mRNA in control group, tyrosine group I and tyrosine group I1 were 1.11 ± 0.36, 2.08 ± 0.45, 2.52 ± 0.65 and 1.21 ±0.36, 1.94 ± 0.57, 2.10 ± 0.56, respectively. The differences were statistically significant（F = 10.48, 4.42, all P 〈 0.05）. The mRNA levels of TG, NIS in tyrosine group I and tyrosine group Ⅱ were significantly higher than that of control group（all P 〈 0.05）. TPO, TSHR mRNA expression levels of control group, tyrosine group I and tyrosine group 1I were 1.02 ± 0.54, 1.14 ± 0.29, 1.17 ± 0.38 and 0.81 ± 0.44, 0.91 ± 0.30, 0.90 ± 0.19, respectively. There was no significant differences among the three groups on TPO, TSHR mRNA levels （F = 0.18, 0.26, all P 〉 0.05）. Conclusions Tyrosine could effect on expression of thyroid cell function related gene and increase the storage of iodine in the thyroid by stimulating the iodine pump function, which may increase iodine intake.