摘要
目的:观察sPD-1蛋白对小鼠H22肝癌腹水瘤生长的抑制作用。方法:建立H22肝癌小鼠腹水瘤模型,将24只接种H22肝癌细胞BALB/c小鼠随机分成3组,其腹腔分别注射PBS(模型组)、DDP(阳性对照组)以及sPD-1蛋白,用药15 d后,停止用药,取腹水测定腹水细胞Treg细胞的产生情况,并观察小鼠的毒副反应以及生存期。结果:sPD-1蛋白组的H22肝癌生命延长为(25.75±4.30)d,DDP组的H22肝癌生命延长为(25.00±4.41)d,均明显高于对照组的(16.63±2.67)d(P<0.05)。sPD-1蛋白组小鼠腹水中Treg细胞明显少于DDP组和模型组,免疫水平优于DDP和PBS组。结论:原核表达的sPD-1蛋白对小鼠H22肝癌腹水瘤的生长具有明显抑制作用,并能促进机体免疫系统功能,提高生存期。
Objective: To research the inhibitory effect of sPD-1 protein on H22 liver cancer in mice. Methods: The mouse hepatoma H22 cells were injected intraperitoneally into the BALB/c mice to establish H22-bearing mice model, and all the 24 mice H22-bearing mice were randomized into three groups according to different treatment: PBS group (model group), DDP group (positive control), and sPD-1 protein group. After 15 days' treatment, ascites samples were taken out from the tumor bearing mice for Treg cells detection, and survival days were compared. Results: The life prolonged days in sPD-1 group (25.75±4.30) and DDP (25.00±4.41) group were significantly longer than that in control group (16.63±2.67) (P〈0.05). Compared with that in DDP group and model group, Treg cells in ascites of sPD-1 protein group were significantly less. Conclusion: sPD-1 protein has an anticancer effect on H22 liver cancer in mice, and can promote the immune function and prolong the survival time.
出处
《武汉大学学报(医学版)》
CAS
北大核心
2013年第1期28-30,共3页
Medical Journal of Wuhan University