摘要
目的探讨补体Clq对Aβ纤维诱导的小胶质细胞炎性反应的影响。方法在一定浓度Aβ纤维刺激的基础上，向BV-2小胶质细胞中加入不同浓度Clq，并且利用C1qA竞争性与Ap的结合以完成对比实验，利用夹心酶联免疫吸附法（ELISA）测定上清液及细胞裂解液中白介素（IL）-6和肿瘤坏死因子（TNF）-α的浓度。结果在AB纤维刺激下，加入50nmol／L补体Clq后，上清液TNF-α浓度较单独加入Aβ纤维明显增加（F=1177．270，P〈0．05）；但在此基础上加入补体ClqA后，TNF-α浓度降低（P〈0．05）。除阳性对照组外，各实验组上清液及细胞裂解液中IL-6浓度相互比较差异无统计学意义（均P〉0．05）。结论补体Clq可能通过促炎性因子TNF-α增强了Aα诱导的BV-2小胶质细胞的炎性反应。
Objective The impact of complement Clq on inflammation in beta amyloid- stimulated microglia. Methods After the cultured BV-2 microglial cells were treated with 100 mg/L betaamyloid fibers （fAβs）, some of them were given Clq, others were given Clq and ClqA. Then, interleukin-6 （IL-6） and tumor necrosis factor a （TNF-α） in the supernatant and cell lysate were determined by the sandwich ELISA. Results A significant increase in TNF-α started at giving 50 nmol/L Clq after 100 mg/L fAβs（F= 1177.27, P〈0.05）, while the release of TNF-α was significantly suppressed by using 50 nmol/L ClqA on basis of this（P〈0.05）. The level of IL-6 showed no above change. Conclusions Clq may enhance the inflammation of Aβ-induced BV-2 microglia cells and TNF-α may play important role in this effect.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2013年第1期96-98,共3页
Chinese Journal of Geriatrics
基金
广东省医学科学基金（C2009031）
广州市科技计划项目（2010Y1-C101）
