摘要
目的观察外源基因人B型利钠肽对慢性心力衰竭大鼠心肌细胞凋亡因子及Ⅰ、Ⅲ型胶原表达的影响。方法 30只慢性心力衰竭模型大鼠,随机分为携带人B型利钠肽基因重组腺病毒组(Ad-hBNP组,14只)、重组空白腺病毒组(Ad-Track组,8只)、生理盐水组(NS组,8只),分别给予腹腔注射人B型利钠肽基因重组腺病毒、重组空白腺病毒及生理盐水1mL,每周1次,共4周。另设10只未造模但实行了假手术的大鼠为假手术组;4周后处死大鼠,行RT-PCR检测心肌组织Bcl-2、Bax、Caspase-3及Ⅰ、Ⅲ型胶原mRNA含量。结果 Ad-hBNP治疗后,Ad-hBNP组大鼠心肌组织Bcl-2mRNA表达较Ad-Track组及NS组升高;BaxmRNA表达较假手术组升高;Caspase-3mRNA表达较Ad-Track组、NS组及假手术组均升高。Ad-hBNP组Ⅰ、Ⅲ型胶原mRNA表达较Ad-Track组降低。结论间断给予Ad-hBNP能够影响慢性心力衰竭大鼠心肌细胞凋亡相关基因的表达,减少心肌细胞间质Ⅰ、Ⅲ型胶原的表达。
Objective To observe the effect of recombinant adenovirus Ad-hBNP on the expression of apoptotic factors and collagen typeⅠ＆Ⅲ of cardiac myocytes in rat model with chronic heart failure （CHF）. Methods Thirty CHF rats were randomly divided into Ad-hBNP group （n=14）, Ad-Track group （n=8）, normal saline group （NS, n=8）. The human B-type natriuretic peptide gene recombinant adenovirus, recombinant blank adenovirus and saline 1 mL were given intraperitoneally, once a week for a total of four weeks. Ten sham-operated rats served as control group. After 4 weeks treatment, the mRNA expressions of Bcl-2, Bax, Caspase-3 and collagen type Ⅰ＆Ⅲ were detected by RT-PCR. Results The mRNA expression of Bcl-2 and caspase-3 were increased in Ad-hBNP group （2.70±1.86 and 0.75±0.30） than those in the Ad-Track group （0.49±0.02, P 〈 0.01 and 0.43±0.26, P 〈 0.05） and NS group （0.95±0.17, P 〈 0.05 and 0.37±0.16, P 〈 0.05）. The mRNA expression of Bax was increased in Ad-hBNP group （0.86±0.17） than that of sham group （0.70±0.07,P 〈 0.05）. The mRNA expression of collagen type Ⅰ＆Ⅲ was decreased in Ad-hBNP group than that of Ad-Track group. Conclusion Exogenous hBNP affected the mRNA expression of apoptotic factors in cardiac myocytes of rat model with chronic heart failure, also reduced the mRNA expression of collagen type Ⅰ＆Ⅲ .
出处
《天津医药》
CAS
北大核心
2013年第1期44-47,共4页
Tianjin Medical Journal
基金
天津市卫生局科技基金项目(项目编号:07KY01)
