洋葱胞质雄性不育系及其保持系花蕾内源激素含量和脯氨酸含量的动态变化特征

Dynamic Changes of Endogenous Hormones and Free Proline in Cytoplasmic Male Sterile Lines and Maintainer Lines of Onion(Allium cepa L.)

以洋葱胞质雄性不育系63A及其同核异质保持系63B为试材,研究了花蕾发育过程中IAA、GA3、ZR、ABA含量以及脯氨酸含量的动态变化规律,探讨洋葱不育性与内源激素、脯氨酸含量的关系。结果显示,洋葱不育系花蕾的IAA、GA3、ZR和脯氨酸含量在败育过程中显著低于保持系,而ABA含量则较保持系有不同程度的盈积。且在花药的发育过程中,二者的差异主要发生在单核期,即不育系小孢子败育的主要时期。据此认为,IAA、GA3、ZR、ABA和脯氨酸含量的异常与洋葱雄性不育的发生有关。

Onion（Allium cepa L.） buds of cytoplasmic male-sterile（CMS）line and its maintainer line at different developmental stages were employed as experimental materials to reveal relationships between CMS and changes of endogenous hormone and prolin contents by measuring IAA,GA3,ZR,ABA and free prolin contents.The results showed that in course of abortion,sterile buds were lower significantly in IAA,GA3,ZR and free prolin contents,while higher in ABA contents.In anthers development stages,the difference happenned mainly in the main pollen abortion stage（monokaryophase）.It is suggested that the contents of IAA,GA3,ZR,ABA,and free prolin may be related with the occurrence of male-sterile onion.