滋阴活血方对自发性高血压大鼠氧化应激反应的影响

Effect of Ziyinhuoxue Decoction on Oxidative Stress of Spontaneously Hypertension Rats

目的观察滋阴活血方对SHR大鼠氧化应激反应的影响。方法将SHR大鼠50只随机分为空白组、对照组、滋阴活血方低、中、高剂量组,每组各10只。对空白组以生理盐水灌胃,对照组以贝那普利灌胃,对剩余3组分别以低、中、高剂量中药灌胃,4周后检测5组大鼠心、肾组织p22phox蛋白表达进行比较。结果对照组、滋阴活血方组较空白组心、肾组织p22phox蛋白降低,滋阴活血方中、高剂量组无明显差异。结论滋阴活血方对自发性高血压大鼠心肾组织中p22phox蛋白表达具有抑制作用,但作用不及贝那普利。滋阴活血方对p22phox蛋白表达的抑制作用与组方剂量存在一剂量曲线,到一定剂量后量效关系不再是正线性相关。

Objective： To observe the effect of Ziyinhuoxue Decoction on Oxidative stress of Spontaneously Hypertension Rats. Methods： Fifty SHR rats were randomly averagely divided into five groups：blank group, Control group,low dose TCM treatment group,medium dose TCM treatment group,high dose TCM treatment group. The Control group were administered intragastrically with benazepril, and the TCM group were administered intragas- trically with Ziyin Huoxue Decoction of different density. After 4 weeks,all rats were executed the protein of p22phox in hearts and kidneys. Results：Compared with the blank group,the proteins of p22phox were lower in the other groups.There was no difference in medium dose grop , compared with high dose grop. Conclusion： Ziyinhuoxue Decoction can lower the p22phox protein in hearts and kidneys of SHR,which is not as good as benazepril. There is a dose curve between the inhibition effect on expression of p22phox protein and the dose of Ziyinhuoxue Decoction, and the dose-effect relationship does not appear linear positively.