高效液相色谱法测定果粒橙中维生素C的含量

Analysis of Vitamin C in Orange by HPLC

建立了反相黼效液相色谱（HPLC）测定果粒橙中维生索C的测定方法。采用LC-100PLUS液相色谱仪，AgilentTC-C18色谱柱（4．6×250mm，5μm）．以0．05mol／L磷酸二氢钾：甲醇=96：4（VN）作流动相，室温25℃，流速0．6mL／min，检测波长288nm。维生素C浓度与峰面积在5～80μg／mL的范围内呈现良好的线性关系，线性回归方程为y=11．2534x-17．0162（R=0．9989）。相对标准偏差为1．40％。该测定方法操作简便、灵敏、准确、可靠，适用于多数饮料中维生素C含量的测定。

A method has established for the detection of Vitamin C （Vc） in orange juice by high performance liquid chromatography （HPLC）. The LC-100PLUS HPLC system with Agilent TC-C18 column （4.6×250 mm, 5 μm） was used. The samples were analyzed using 0.05 mol/L potassium dihydrogen phosphate： methanol （V：V=96：4） as mobile phase with the flow rate of 0.6 mL/min. The temperature of the column was room temperature and the detection wave length was 288 nm. The standard working curve of Vc and peak area had a good linear relation between the concentration from 5 μg/mL to 80μg/mL. The linear equation was y=1 1.2534 x-17.0162and the related coefficient equaled to 0.9989. The relative standard deviation of this method was 1.40 %. This method proved to be a simple, rapid, exact and reliable way to determine the content of Vitamin C in most of the beverages.