气相色谱法同时测定烟叶中三种农药的残留

Determination of 3 Pesticide Residues in Tobacco by Gas Chromatography

采用气相色谱法研究了仲丁灵、氯氰菊酯、溴氰菊酯3种农药在烟叶中的残留情况。样品经乙腈提取,Florisil硅土和中性氧化铝双吸附剂层析柱净化,正己烷-乙酸乙酯(98∶2,V/V)洗脱剂洗脱和浓缩后,采用GC-ECD进行定性和定量分析。当样品的加标水平为0.05,0.25,1.0 mg/kg时,加标回收率为73.8%～89.4%,相对标准偏差在5.5%～10.3%之间。该方法可用于烟叶中农药残留的快速分析测定。

A method was developed for rapid determination of 3 pesticide residues in tobacco based on gas chromatography. After the optimization of different parameters, such as the extraction solvent, elution solvent, purificant, and pesticides were extracted from tobacco with acetonitrile, cleaned - up on a column, packed with Florisi and neutral alumi- na, eluted with hexane - ethyl acetate （98 ： 2, V/V）, and then determined by GC - ECD. The recoveries were per- formed at O. 05, 0.25 and 1.0 mg/kg fortification levels for each pesticide, and the recoveries ranged 73.8% - 89.4% with the relative standard deviations between 5.5% - 10.3%. Therefore, this method can satisfy the requirements for the rapid analysis of pesticides in the tobacco.