自拟地圣珠水煎剂对血管性痴呆小鼠海马IL-1β、IL-6的影响

Effects of Chinese compound recipe Dishengzhu on IL-1β and IL-6 in brain of synthetic vascular dementia mouse

目的观察自拟地圣珠水煎剂对血管性痴呆(VD)模型小鼠脑海马组织中IL-1β、IL-6的影响。方法 45只小鼠随机分为假手术组、模型组、中药组各15只,后2组采用夹闭小鼠双侧颈总动脉造成脑缺血再灌注脑损伤模型,而后中药组给予地圣珠水煎剂,模型组、假手术组给予等量生理盐水,2周后采用ELISA法测定小鼠模型脑海马组织中IL-1β、IL-6的水平。结果与假手术组相比,模型组小鼠脑海马IL-1β、IL-6水平显著升高(P<0.05),而与模型组比较,中药组IL-1β、IL-6水平明显降低(P<0.05)。结论地圣珠水煎剂能够通过下调脑海马组织中IL-1β、IL-6的水平,干扰炎性反应继发损伤过程,从而达到对VD的满意干预效果。

Objective To observe the effects of Chinese compound recipe Dishengzhu on the level of 1L-1βand IL-6 in brain hippocampal tissue of mouse models with vascular dementia.Methods 45 mouses were divided into 3 groups randomly and averagelv including the pseudo-operation group,the model control group and the Dishengzhu group,duplicated the synthetic vascular dementia mouse models through transient occlusion of bilateral carotid artery in the model control group and the Dishengzhu group.The Chinese medicine group is administed respectively with the compound recipe Dishengzhu while other groups with the normal saline only.The level of IL-1βand IL-6 in brain hippocampal tissue of mouse models was observed by the means of ELISA method 2 weeks later.Results Compared with the pseudo-operation group,the level of IL-1βand IL-6 in brain hippocampal tissue of mouses was improved apparently in the model control group（P 0.05）.Compared with the model control group,the level of IL-1 B and IL-6 was markedly decreased in brain hippocampal tissue of mouses in Dishengzhu group （P 0.05）.There were remarkable differences.Conclusion The curative effect of Chinese compound recipe Dishengzhu was remarkable by the mean of decreasing the level of IL-1βand IL-6 in brain hippocampal tissue and restraining inflammatory response.