摘要
以干旱胁迫下丙二醛含量作为抗旱性鉴定指标,对K55×K58杂交组合的亲本、F1、F2:3家系丙二醛分析结果表明:自交系K58为强抗旱性品种,K55为弱抗旱性品种,F1为高抗,F2:3家系抗、感分离比例符合3:1。进一步利用BSA法对60对SSR标记进行筛选,其中引物ORS510和ORS317在抗感基因池间表现稳定的多态性,并且在F2、F2:3家系个体中表现出抗性与多态性协同分离的趋势。采用MAPMAKER数据分析软件分析,表明ORS510、ORS317距离干旱胁迫下控制丙二醛含量的抗性基因分别为6.8cM、9.7cM。
We studied on the cross combinational parents of the K55 ×K58 and F1,F2：3 family of malondialdehyde content as drought-resistance identification indexes under drought stress results showed that K58 had drought resistance,K55 had emotional variety,F1 had the high resistance and F2：3 family resistant and susceptible separation in line with the 3：1 ratio.Using BSA method of 60 pairs SSR markers screening,including primer ORS510 and ORS317 in anti-influenza gene pool had stable performance and in the F2,F2：3 family individual showed resistance and polymorphism collaborative separation trend.Depending on the software MAPMAKER data analysis showed that ORS510 and ORS317 distances from the MDA chain gene-related sites were 6.8cM,9.7cM under drought stress.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
北大核心
2012年第3期25-29,共5页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
内蒙古自治区自然科学基金重点项目(编号:2010zd06)
国家科技支撑计划项目(2009BADA8B04)
关键词
油葵
丙二醛
SSR分子标记
Oil sunflower
Malondialdehyde
SSR molecular markers
