亚硝酸钠在失血性休克复苏中对肺组织的保护作用

目的探讨亚硝酸钠在失血性休克时对肺组织的保护作用及可能机制。方法 SD大鼠24只随机分为3组(n=8),即对照组、休克复苏组和亚硝酸钠治疗组。对照组只做动脉血压监测,其余两组在休克2 h后回输全部失血和等量无菌盐水,亚硝酸钠治疗组在休克复苏1 0min时静脉给药4.8 mmol/L亚硝酸钠溶液100μL,对照组和休克复苏组给予等量无菌生理盐水。健康SD大鼠失血性休克2 h,复苏1 h处死,取肺组织测定一氧化氮(Nitric Oxide,NO)含量及一氧化氮合酶(Nitric oxide synthase,NOS)活性,观察肺组织病理变化。结果休克复苏组肺组织NO含量、NOS活性明显高于对照组和休克复苏组,对照组NO含量和NOS活性最低;对照组肺组织病理变化最轻,接近正常肺组织,亚硝酸钠组肺组织病理变化比休克复苏组明显减轻。结论亚硝酸钠可明显减轻大鼠失血性休克复苏时对肺组织的损伤,其机制可能是通过某种机制抑制NOS的激活,避免产生大量NO从而减轻肺组织损伤。

Objective To study the protective effect of Sodium nit- rite （SN） on lung injury induced by hemorrhagic shock-reperfusion in rat. Methods To randomLy divide on average 24 healthy SD rats into Operation control group （Group OC）, hemorrhagic shock resuscitation group （Group HSR） and Sodium nitrite group （Group SN）.When the experiment finishes, select lungs to determine the activity of NO and NOS,and make a pathological investigation of the lungs.Results To be comparedwith groupHS,NO content and NOS activity in lungs in group SN increase drastically;To be compared with group HS pathological changes of lungs in group SN apparently weaken.Conclusion SN plays a good and protective role in lung injury.Likely, SN has a protective effect on lung reperfusion injury through reduccing NOS activity.After rats hemorrhagically shocking resuscitate, NO emits numerously to participate in the course of lung daminification.