SYBR Green荧光定量PCR法对BCL11B基因座位相互作用位点的验证分析

SYBR Green-based quantitative PCR analysis on the BCL11B locus interaction

目的:研究在不同类型细胞核中,位于不同染色体上的BCL11B与ARHGAP6基因座位间的相互作用关系。方法:通过SYBR Green荧光实时定量PCR法,分别对MCF-7和Jurkat细胞的3C(染色体构象捕获)样品进行定量分析,分析分别位于不同染色体上的BCL11B与ARHGAP6基因座位间的相互作用频率。结果:与TaqMan探针法相比较,基于SYBR Green荧光实时定量PCR法对3C样品的分析同样具有良好的特异性。在MCF-7和Jurkat细胞中,BCL11B与ARHGAP6基因座位间的相互作用频率存在很大的差异。结论:SYBR Green荧光实时定量PCR法在染色体构象捕获技术中具有很好的特异性;BCL11B与ARHGAP6基因座位间的相互作用具有细胞类型特异性。

Objective. To study the interaction relationship of the iner-chromosome BCLllB and ARHGAP6 loci in different types of cell nuclei. Method： With SYBR Green-based real-time quantitative PCR assays, we analyzed chromosome conformation capture （3C） samples of MCF-7 and Jurkat cells to detect the interaction frequencies between t the iner-chromosome BCLllB and ARHGAP6 loci in different types of cell nuclei. Result： Compared with the TaqMan probe-based quantitative PCR assays, SYBR Green-based real-time quantitative PCR assay displayed the special amplification results with the 3C DNA samples. In MCF-7 and Jurkat cells, there were different interaction frequencies between the BCLllB and ARHGAP6 loci. Conclusion= The SYBR Green-based real-time quantitative PCR assay can reveal special amplification results with the template of 3C DNA samples. There were cell-type interaction frequenciesbetween the BCLllB and ARHGAP6 loci.