反相高压液相色谱与荧光分光光度计朕机检测儿茶酚胺类物质

DETERMINATION OF DA,NE AND E BY REVERSED-PHASE HPLC AND FLUORESCENCE SPECTROPHOTOMETRY

本文就高效液相与荧光分光光度计联机检测多巴胺、去甲肾上腺素、肾上腺素的方法加以论述。

Dopamine (DA) , norepinephrine (NE) and epinephrine (E) are neuro-transmitters present in the body in very low concentration. Many diseases can induce changes in the concentration of them in tissues and urine. Using HPLC and fluorescence spectrophotometry, their concentration can be directly determined without derivation. The advantages of this method are small sample size and high accuracy.