红湘莲莲皮粉果胶多糖的提取工艺研究

Study on Extraction Conditions of Pectin from Lotus (Nelumbo nucifera ‘Hong Xiang Lotus’) Seed Peel Waste

用草酸铵溶液作为提取溶剂提取了红湘莲莲皮粉中的果胶多糖。通过单因素实验法考察了草酸铵浓度、提取温度、提取时间以及料液比等四个因素对果胶多糖得率的影响。在单因素实验的基础上,通过正交实验,得到的最佳工艺条件为:草酸铵浓度0.7%,提取温度100℃,提取时间2 h,料液比1:30(m/V)。在该条件下,果胶多糖的得率为12.50%。所得果胶多糖经FTIR分析和酯化度测定为低酯化度果胶。

Pectin polysacchaxide was extracted from lotus （Nelumbo nucifera ＇Hong Xiang Lotus＇） seed peel waste by using ammonium oxalate aqueous solution as the extraction solvent. Effects of extraction conditions such as extracting temperature, ratio of solid to solvent, concentration of ammonium oxalate and extracting time on the extraction yield were studied by single factor analysis method. The orthogonal experiment design was used to optimize the extraction conditions. The optimal extraction conditions are as follows： extracting temperature 100 ℃, ratio of solid to solvent 1：30 （m/V）, concentration of ammonium oxalate 0.7% and extracting time 2 h. Under these conditions, the extraction yield 12.5% is highest. The obtained extract was identified as low methoxyl pectin by fourier transform infrared spectroscopy （FTIR） and determination of the degree of esterification （DE）.