同一细胞DNA和RNA同时分别提取的简便方法

A SIMPLE METHOD FOR EXTRACTION OF DNA AND RNA FROM THE SAME CELL

本文探讨并建立了从同一细胞中同时提取细胞核DNA和细胞浆RNA的方法。经紫外分光光度仪扫描,生化测定以及电泳等方法的鉴定表明:提取的核酸无论从量还是从质上均可满足对DNA和RNA的各种实验研究。此法简便稳定,是从同一细胞中分别提取DNA和RNA的理想方法。

We established a simple method for extraction of DNA from the nucleus and RNA from the cytosol of the same cultivated'cells or cell lines. The quality and quantity of DNA and RNA thus extracted, identified by means of spectrophoto-metric scanning, biochemical assay and electrophoresis, are suitable for various researches. Experiments showed that this is an ideal method for simultaneous extraction of DNA and RNA from the same cells.