腺嘌呤制作尿酸性肾小管损害SD大鼠模型的研究

Study on the Production of Uric Acid Renal Tabular Damages in SD Rat Model by Using Adenine

目的腺嘌呤灌服法制作符合尿酸性肾病的SD大鼠模型。方法给SD大鼠以腺嘌呤按200mg/(kg d),第1～12d每日1次,第13～30日隔日一次灌服给药法喂饲,开始给药后每日观察临床表现,并设生理盐水对照组、空白对照组,第31d经麻醉后处死,取左肾组织做病理切片观察。结果大鼠肾脏变化主要围绕尿酸盐结晶所引起的肾小管炎性改变,病变主要为肾小管炎性水肿坏死,并累及肾间质、肾小动脉。结论上述腺嘌呤灌服法造成准确的以肾小管损坏为主,并见肾间质、肾小动脉病变的SD大鼠模型,类似于人类的尿酸性肾病所致的肾脏原发性病理改变及继发性病理变化。

Objective To produce SD rat model with uric acid nephropathy basic pathological changes by feeding with adenine.Method To use improved adenine 200mg/kg to feed the SD rat for 1st～12thdays once a day,the 13th～30th day two days once.After medication,perform daily clinical observation.At the same time,to set saline group and blank group.On 31st day,all rats were sacrificed with anesthesia and take the left kidney for pathology slice observation.Results The kidney renal pathology changes mainly focus in the process of crystalisation of uric asid in renal tabular which cause inflammatory changes.The pathology changes is mainly in the gradually lesions in renal tabular until damage and involving the renal interstitial renal artery.Conclusion By using adenine as above and irrigation feeding,can produce SD rats with main focus in renal tubular damage,with renal interstitial and renal artery pathology changes,similar to the human with uric acid nephropathy which cause the kidney failure with primary and secondary pathological changes.