摘要
目的改进新生大鼠低氧缺血性脑损伤(HIBI)模型的制作方法,观察低氧缺血对脑组织病理形态和神经营养因子的影响。方法大鼠随机分为空白对照组(n=5)、假手术组(n=8)和HIBI模型组(n=19),HIBI模型制作中省去了经典Rice法中的麻醉步骤和动物手术后休息时间,观察HIBI后大鼠体重增长情况,行为能力表现以及脑组织病理形态学改变;比较HIBI制模后3 d假手术组及HIBI组鼠脑匀浆beta-NGF和human-NT3的变化。结果 (1)HIBI模型组体重增长明显落后于空白对照组和假手术组(P<0.01);(2)HIBI组全部出现不同程度的行为异常:84%翻身不能,63%肌肉颤动和/或头颤,抽搐者占42%,死亡率为21%。制模后3 d HIBI模型组大鼠的行为障碍和异常运动的发生率均明显低于制模当日(P<0.01);(3)HE染色可见HIBI模型组大鼠左侧大脑半球神经元损伤及神经胶质细胞增生;(4)制模后3 d鼠脑匀浆human-NT3含量较假手术组增加(P<0.05);β-NGF含量无明显变化。结论制作的新生大鼠HIBI模型更符合临床新生儿HIBI的自然病程。HIBI早期神经营养因子表达增加在神经保护机制中发挥重要作用。
Objective To improve a model of hypoxic-ischmic brain injury (HIBI) in neonatal rats and observe the effects of hypoxic-ischemic brain injury on cerebral pathology and neurotrophic factor. Methods Thirty-two healthy 7- day-old neonatal Sprague-Dawley (SD) rats were used as research subjects and randomly divided into 3 groups: the control group (n =5), the sham operated group (n =8), and the HIBI group (n = 19). The rats in the HIBI group received hy- poxia immediately after operation without previous anaesthesia. The body weight, behavioral ability and neuropathological changes in each group were observed. β-NGF and human-NT3 of the brain tissue in the sham operated and HIBI groups were compared on d3 and d14. Results ( 1 ) The body weight gain of the HIBI group was significantly slower and lower than that in the control group and sham operated group (P 〈 0.01 ). (2) Behavioral abnormalities were found in all rats of the HIBI group including unable to turnover (84%), muscle tremor and/or head tremble (63%) and mortality (21%).(3) HE staining showed neuronal damages and proliferation of neuroglial cells in the left hemisphere of the HIBI group. (4) The concentration of human-NT3 in the HIBI group was increased than that of sham operated group, but the concentra- tion of β-NGF was not significantly different from that in the two groups on d3. Conclusions A neonatal rat model of HIBI is successfully established and it is more closely resembling the course of human neonatal HIBI. The increase of neurotro- phic factor plays an important role in the neuroprotection mechanism in the early phase of HIBI.
出处
《中国实验动物学报》
CAS
CSCD
2012年第6期47-50,I0008,I0009,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
北京市科技计划(D07050701350704)
中日友好医院院级课题(编号2010-MS-03)
关键词
缺氧缺血
脑
新生
大鼠
神经营养
Hypoxic-ischemic brain injury
Neurotrophic factor
neonatal rats