不同品系大鼠RTI基因抗原表达数量的研究

Expression of RTI Gene Antigen in Different Rat Strains

目的研究不同品系大鼠RT1A、RT1B和RT1D基因表达的抗原总数量,以及在CD4+T细胞和CD8+T细胞上表达的数量,为免疫学研究提供数据支持。方法采集四种品系BN、Lewis、F344、SHR大鼠的静脉血,制备淋巴细胞,与有荧光标记的单克隆抗体反应,应用流式细胞术检测抗原数量。结果发现RT1A、RT1B和RT1D基因表达的抗原在不同品系大鼠体内表达的数量不同,其中F344大鼠表达的RT1A抗原最多,BN大鼠表达的RT1B抗原和RT1D抗原均为最多。RT1A、RT1B和RT1D基因表达的抗原在CD4+T细胞和CD8+T细胞上表达的数量也不同,Lewis大鼠在CD4+T细胞上表达的RT1A抗原最多,BN大鼠在CD4+T细胞上表达的RT1B和RT1D抗原最多。F344大鼠在CD8+T细胞上表达的RT1A抗原最多;F344大鼠在CD8+T细胞上表达的RT1B和RT1D抗原最多。同一品系大鼠之间雌雄动物RT1A、RT1B和RT1D基因表达的抗原也不同。结论不同品系大鼠RT1A、RT1B和RT1D基因的抗原总表达数量之间差异有显著性,在CD4+T细胞上和CD8+T细胞上表达的数量差异也有显著性。

Objective To study the total amount of expression of RT1A,RT1B and RT1D gene antigens in different rat strains,their amount of expression in CD4＋ T cells and CD8＋ T cells,and provide support data for immunology researches.Methods Venous blood was collected from the rats of BN,Lewis,F344,SHR strains.Lymphocytes were isolated,and reacted with fluorescent marker of monoclonal antibody.The amount of gene antibodies was assessed by flow cytometry.Results The RT1A,RT1B and RT1D gene antigen expressions were different in different rat strains.Among them,the expression of RT1A antigen was highest in F344 rats,and expression of RT1B and RT1D gene antigens was highest in BN rats.The amount of expression of RT1A,RT1B and RT1D gene antigens on CD4 ＋ and CD8＋ T cells were also different.Among the studied rat strains,the expression of RT1A antigen on CD4＋ T cells was highest in Lewis rats,and the expression of RT1B and RT1D gene antigens on the CD4＋ T cells was highest in BN rats..The expression of RT1A antigen on CD8＋ T cells was highest in F344 rats,and the expression of RT1B and RT1D gene antigens on CD8＋ T cells was highest in BN rats.A higher expression of RT1A,RT1B and RT1D gene antigens was found in the male rats than in female rats of the same strain.Conclusions There are significant differences between the total amounts of expression of RT1A,RT1B and RT1D gene antigens in different rat strains,and also significant differences between the amounts of their expression on CD4＋ and CD8＋ T cells in these rat strains.Our results indicate that it is important to choose appropriate rat strain to provide reliable and proper results in immunological researches.