HIV感染后不同病程阶段淋巴结中CD4^+T淋巴细胞各亚群频率的改变

Changes of the frequencies of CD4^+ T lymphocyte subsets in lymph nodes in patients with different stages of HIV infection

目的评价淋巴结(lymph nodes,LNs)中初始、中枢记忆性、效应记忆性和效应CD4+T淋巴细胞频率的改变,探讨伴随疾病进展,LNs中CD4+T淋巴细胞各亚群频率改变的可能原因。方法选取HIV感染者71例,根据外周血CD4+T淋巴细胞计数和临床症状,将其分为HIV感染无症状组和AIDS患者组,并选取28例非HIV感染者作为健康对照组,留取以上3组LNs组织行活体组织检查,分离其中淋巴细胞,利用流式细胞术检测各细胞群的频率。结果①AIDS患者组LNs中初始CD4+T淋巴细胞频率与无症状组及健康对照组相比显著升高,而无症状组LNs中初始CD4+T淋巴细胞频率与健康对照组相比下降,但差异无统计学意义;②AIDS患者组LNs中中枢记忆性CD4+T淋巴细胞频率与健康对照组相比显著升高。虽然无症状组LNs中中枢记忆性CD4+T淋巴细胞频率与AIDS患者组相比下降,与健康对照组相比升高,但差异均无统计学意义;③AIDS患者组、无症状组和健康对照组LNs中效应记忆性CD4+T淋巴细胞频率依次升高,3组相比差异均有统计学意义;④无症状组、AIDS患者组和健康对照组LNs中效应CD4+T淋巴细胞频率依次降低,但3组相比差异均无统计学意义。结论 HIV感染后,LNs中初始、中枢记忆性、效应记忆性和效应CD4+T淋巴细胞频率并非随着疾病进展进行性下降。

Objective To investigate the changes of the frequencies of na＇fve, central memory, effector memory and effector CD4＋ T lymphocytes in lymph nodes （LNs） in different stages of HIV infection and explore the potential causes of the changes of CD4＋ T lymphocyte subsets in LNs with disease progression. Methods Seventy-one HIV-infected individuals were included in the study, and were divided into 2 groups, asymptomatic HIV carrier group and AIDS patient group, based on CD4＋ T lymphocyte counts and clinical symptoms, and 28 healthy individuals were recruited as a control group. LNs biopsy of the 3 groups was performed with surgical procedures, lymphocytes were isolated, and the frequencies of CD4＋ T lymphocyte subsets were detected by flow cytometry. Results The frequency of naive CD4＋ T lymphocytes in LNs of the AIDS patient group increased significantly as compared with the asymptomatic HIV cartier group and the control group, and that of the asymptomatic HIV carrier group decreased as compared with the control group, but the difference was not significant. The frequency of central memory CD4＋ T lymphocytes in LNs of the AIDS patient group increased significantly as compared with the control group. Though the frequency of central memory CD4＋ T lymphocytes in LNs of the asymptomatic HIV carrier group was lower than that of the AIDS patient group and higher than that of the control group, the differences were not significant. The frequencies of effector memory CD4＋ T lymphocytes in LNs of the AIDS patient group, the asymptomatic HIV carrier group and the control group increased in turn, and significant differences were found among the 3 groups. The frequencies of effector CD4＋ T lymphoeytes in LNs of the asymptomatie HIV carrier group, the AIDS patient group and the control group decreased in turn, but significant differences weren＇t found among the 3 groups. Conclusion The frequencies of naive, central memory, effector memory and effeetor CD4＋ T lymphocytes in LNs do not progressively decrease with disease progression after HIV infection.