贵州省侗族人群mtDNA 9 bp序列缺失频率及DYS287位点多态性研究

Study on Frequency of 9 bp Sequence Deletion of Mitochondrial DNA and Polymorphism of DYS287 Site in Dong Minority of Guizhou Province

目的:分析贵州省侗族人群线粒体DNA 9 bp序列缺失频率及Y染色体DYS287位点的多态性。方法:用PCR-聚丙烯酰胺凝胶法(PAGE)分析mtDNA 9 bp缺失多态性,DNA序列分析法验证结果;用PCR-琼脂糖凝胶电泳检测法分析DYS287位点多态性。结果:在96例侗族男性个体中,mtDNA 9 bp缺失多态频率为13.54%(13/96),DYS287全部显示为YAP-。结论:获得贵州省侗族人群mtDNA 9 bp序列缺失频率及Y染色体DYS287位点多态数据,为该民族群体遗传分析及起源提供了相关遗传背景资料。

Objective： To analyze the frequency of 9 bp sequence deletion of mitochondrial DNA （mtDNA） and the polymorphism of DYS287 site of Y-chromosome in Dong minority of Guizhou prov- ince. Methods： PCR and polyacrylamide gel electrophoresis （PAGE） were used to analyze mtDNA 9bp sequence deletion polymorphism. PCR and agarose gel electrophoresis were used to detect DYS287 polymorphism. DNA sequence analysis was used to verify the results. Results： In 96 unrelated Dong individuals, the frequency of mtDNA 9bp sequence deletion was 13.54% （ 13/96 ） , and YAP element was absent. Conclusions： The genetic data of mtDNA 9bp sequence deletion frequency and Y-chromo- some DYS287 site polymorphism in Dong minority are obtained.