低浓度丝裂霉素对肺腺癌A549细胞增殖及调控的影响

Effect of Low Concentration of Mitomycin on the Proliferation and Cell Cycle Regulation of A549 Lung Cancer Cell

目的:探讨低浓度丝裂霉素对肺腺癌A549细胞增殖及调控的影响。方法:应用Western-blot及RT-PCR方法检测低浓度丝裂霉素处理后的肺腺癌A549细胞中caspases-3、p21、RARα、nm23、bcl-2、cyclinD3表达,MTT法检测细胞的增殖抑制情况。结果:低浓度丝裂霉素处理后的肺腺癌A549细胞中caspases-3、p21、nm23表达增强,RARα、bcl-2、cyclinD3表达减弱,处理后的细胞出现明显的增殖抑制。结论:低浓度丝裂霉素能够使肺腺癌A549细胞增殖抑制,癌基因表达水平下调,抑癌基因表达上调,多基因表达水平的改变可能参与了肿瘤细胞的分化调节。

Objective： To explore the impact of low concentration of mitomycin on proliferation and regulation of human lung adenocarcinoma cells A549. Methods： Western-blot and RT-PCR methods were used to detect the expression changes of caspases-3, p21, RARct, nm23, bcl-2 and cyclinD3 in lung adenocarcinoma ceils A549 after treating with low concentration of mitomycin. Results： The ex- pression Caspases-3, p21 and nm23 increased in the lung adenocarcinoma cells A549 after low concen- trations of mitomycin handling, while RARα, bcl-2 and cyclinD3 decreased. Conclusions： The low concentration of mitomycin enables the proliferation and expression of lung adenocarcinoma cells A549 to change. It leads to cell prolifation inhibition, low expression of oncogenes and incraesed expression of tumor suppressor genes, and the changes in the expression level of polygenes participate in differen- tiation regulation of tumor cells in general.