青海湖裸鲤精子形态和超微结构的研究

Morphosis and ultrastructure of the spermatozoon of Gymnocypris przewalskii

应用扫描电镜和透射电镜技术,观察了青海湖裸鲤精子的外部形态和超微结构。结果显示,青海湖裸鲤精子由头部、中段和尾部3部分组成。精子头部呈椭圆形,主要结构是细胞核,细胞质极少;核前端无顶体,后端偏向一侧有植入窝,植入窝约凹入细胞核的1/3;核中染色质致密。精子中段由中心粒复合体和袖套组成。中心粒复合体位于植入窝内,包括近端中心粒和基体,近端中心粒和基体之间成钝角关系;袖套与细胞核后端相连,袖套中含有线粒体。精子尾部细长,主要结构是轴丝,为典型的"9+2"型结构,轴丝外两侧为细胞质膜形成的对称性侧鳍。

The morphous and ultrastructural tissues of Gymnocypris przewalskii spermatozoon were observed by scanning and transmission electron microscopes.The spermatozoon consisted of the head,middle piece and the tail.The head of the spermatozoon was oval shape without acrosome,of which the main structure was nucleus,and only little cytoplasma existed.At the caudal-lateral end of the head,implantation fossa trapped into the internal to the 1/3.The chromatin in nucleus was dense.The middle piece included centriolar complex and sleeve.The centriolar complex was inside the implantation fossa,which comprised proximal centriole（PC） and basal body（BB）.The angle between PC and BB was obtuse.The sleeve,which contained several mitochondria,was connected to the posterior end of the nucleus.The tail of the spermatozoon was slim and long.The main composition of the tail was axoneme,whose structure was the typical model of ＂9＋2＂.The tail had lateral fins,which was arranged symmetrically.