冰草10个主要农艺性状的QTL定位研究

Studies on Quantitative Trait Loci of Ten Main Agronomic Traits of Wheatgrass

为了给冰草分子标记辅助育种提供依据,以蒙古冰草、航道冰草及其F2群体为试验材料,在已构建的冰草分子连锁遗传图谱的基础上采用多QTL模型法对茎粗、叶长、叶宽、分蘖数、茎叶比、叶片数、小穗长、单株鲜重、单株干重等10个主要农艺性状进行了QTL定位分析。试验共检测到13个QTL,分布在1、2、3、4、5、7共6个连锁群上,其加性效应各不相同,遗传贡献率变化范围为10.1%～33.9%。在13个QTL中,有1个与株高相关QTL、1个叶长相关QTL,1个单株鲜重相关QTL,2个单株干重相关QTL,1个叶宽相关QTL,1个分蘖数相关QTL,2个茎粗相关QTL,1个叶片数相关QTL,2个茎叶比相关QTL,1个穗长相关QTL。

Quantitative trait loci of main agronomic traits of wheatgrass were studied to provide basis for molecular assisted breeding of wheatgrass.The Agropyron mongolicun,A.cristatum cv.Fairway and their F2 population were used as test materials.Based on the constructed genetic linkage map of wheatgrass,the quantitative trait loci of ten main agronomic traits of stem diameter,leaf length,leaf width,tiller number,ratio of stem to leaf,leaf number,spikelet length,individual fresh weight and individual dry weight were analyzed with the multiple QTL models.Total of 13 quantitative traits loci were detected,which were distributed in linkage group 1,linkage group 2,linkage group 3,linkage group 4,linkage group 5 and linkage group 7.The additive allelic effects were different among the 13 quantitative traits loci with genetic contribution ranged in 10.1%~33.9%.There were a quantitative trait locus related to plant height,a quantitative trait locus related to leaf length,a quantitative trait locus related to individual fresh weight,two quantitative trait loci related to individual dry weight,a quantitative trait locus related to leaf width,a quantitative trait locus related to tiller number,two quantitative trait loci related to stem diameter,a quantitative trait locus related to leaf number,two quantitative trait loci related to ratio of stem to leaf,a quantitative trait locus related to spike length.