冠菌素诱导冬小麦幼苗抗旱性的浓度效应

Effects of Coronatine Concentration on the Induced Drought Resistance in Winter Wheat Seedlings

为检验冠菌素(COR)喷施诱导植物抗旱性的效果,选用两个冬小麦品种济麦22和良星99为材料,分析了不同浓度COR下冬小麦水培幼苗抗旱性的变化。结果表明,在20%PEG-6000的模拟干旱条件下,喷施低浓度(≤1μmol.L-1)的COR增加了小麦幼苗的株高、根长和干物重,提高了叶片叶绿素含量和根系活力,增加了叶片相对含水量、超氧化物歧化酶活性及游离脯氨酸、可溶性糖、可溶性蛋白等渗透调节物质含量;而喷施高浓度(≥10μmol.L-1)的COR溶液则抑制了幼苗生长和生理代谢活性。说明喷施适宜浓度的COR(1～10μmol.L-1)对冬小麦抗旱性有一定的诱导作用。

Coronatine（COR） is one kind of phytotoxin,secreted by Pseudomonas syringae and is similar to the structure of JA,and could induce the plant resistance with the low concentration.The inductive effects on drought resistance of spraying different COR concentrations was studied using two winter wheat cultivars Jimai 22 and Liangxi 99 as experimental materials in a hydroponic experiment.The results shows that,in the condition of 20% PEG-6000 simulated water stress,spraying lower concentration of COR solution（≤1 μmol·L-1） could increase the plant height,root length and dry matter weight of the wheat seedlings,increased the leaf chlorophyll content and root activity,improved the relative water content and the contents of osmosis regulators such as proline,soluble sugar and protein,and increased the SOD activity and decrease MDA content.On the contrary,spraying higher COR solution（≥10 μmol·L-1）would restrain the seedling growth and activities of physiological metabolism.So in conclusion,spraying COR at suitable concentration could increase the drought resistance of wheat.