罗汉果茎尖脱毒技术研究

Study on Stem Apex Detoxification of Siraitia grosvenorii

以罗汉果组培苗为试材,在高温热处理不同天数后剥取茎尖,进行罗汉果花叶病毒脱毒技术研究,同时采用间接ELISA法和电镜观察法检测其脱毒效果。结果表明:适合罗汉果热处理的时间为7～15d,试管苗的成活率可达90%～100%;38.5℃热处理10d剥取0.2～0.5mm的茎尖进行培养,脱毒率为100%。

Siraitia grosvenorii in vitro was used as material in this experiment to eliminate Luohanguo mosaic virus （WMV-2-Luo） by combing tissue Culture of shoot-tip with heat therapy,and detected by indirect ELISA method and electron microscope. The results showed that the suitable time for heat treatment was 7 - 15 days, the survival rate of tissue culture seedling was up to 90%- 100% ;0. 2-0. 5 mm shoot-tip with heat treatment by 38. 513 for 10 days of Siraitia grosvenorii were 100% free from WMV-2-Luo.