摘要
以五味子种子为外植体,研究了不同种类培养基、不同植物生长调节剂、蔗糖及水解酪蛋白对五味子种子萌发和愈伤组织诱导的影响。结果表明:以GA 0.1mg/L、蔗糖20g/L、琼脂5.5g/L为基本培养基时,五味子的种子快速大量萌发,此时,五味子的胚轴诱导愈伤组织出愈率最高为87%,完成愈伤组织的诱导需35d左右。
The effect of culture medium, plant growth regulator, sucrose and casein hydrolysate on the seeds germination and callus induction of Schisandra were studied. The results showed that the seeds of Schisandra had a high frequency regeneration when taking GA 0. 1 mg/L, sucrose 20 g/L, agar 5. 5 g/L as the basic medium. Callus induction of SchisaTMra hypocotyl had the highest rate （87%） than that of other explants. Completion of callus induction took about 35 days.
出处
《北方园艺》
CAS
北大核心
2013年第3期164-167,共4页
Northern Horticulture
基金
吉林省农业科学院引进人才启动基金资助项目
关键词
五味子
组织培养
外植体
愈伤组织
Schisandra chinensis （Turcz） Bail
tissue culture
explant
callus
