小檗碱对梗阻性黄疸大鼠肝细胞的影响

Effect of Berberine on Hepatocytes of Obstructive Jaundice Rats

目的观察盐酸小檗碱灌胃对梗阻性黄疸大鼠肝细胞的影响。方法建立梗阻性黄疸大鼠模型,随机分为正常对照组、假手术组、模型组、小檗碱组,每组8只,小檗碱组给予盐酸小檗碱灌胃。1周后处死,观察肝细胞的凋亡情况、肝组织超氧化物歧化酶(SOD)和丙二醛(MDA)的含量、肝细胞钙含量、血清内毒素水平,同时通过检测血丙氨酸氨基转移酶评价肝功能。结果模型组肝细胞凋亡、细胞内钙离子浓度、MDA含量均较正常对照组以及假手术组升高(P<0.05),而小檗碱组肝细胞凋亡、细胞内钙离子浓度、MDA含量较模型组均明显下降,细胞内SOD含量较模型组明显升高(P<0.05)。结论盐酸小檗碱灌胃对模型大鼠肝细胞有明显的保护作用。

Objective To study the protective effect of berberine administration on hepatocytes of obstructive jaundice rats.Methods Rat model of obstructive jaundice was made.SD rats were randomly divided into normal control group,sham-operation group,model group and berberine group,with 8 rats in each group.The berberine group was given berberine hydrochloride by gavage.After 1 week,the apoptosis of liver cells,SOD and MDA content of liver tissue,the calcium content of liver cells,serum endotoxin level were observed,and serum ALT was detected to evaluate liver function.Results Liver cells apoptosis,intracellular calcium concentration and MDA content of the model group were significantly higher than that of the normal control group and the sham-operation group（P 0.05）.In the berberine group,liver cells apoptosis,intracellular calcium concentration and MDA content were significantly decreased than that of the model group,while SOD content was significantly higher（P 0.05）.Conclusion Berberine hydrochloride has significant protective effect on hepatocytes of model rats.