掌叶半夏脂溶性成分GC-MS研究

GC-MS Analysis on Liposoluble Components of Pinellia pedatisecta

目的:分析掌叶半夏脂溶性成分的化学成分。方法:对乙醇提取后氯仿萃取得到的脂溶性成分进行了GC-MS分析。HP-5MS色谱柱(0.25 mm×0.25 mm×30 m);程序升温,进样口温度280℃,进样量1.0μL,分流比10∶1。结果:共分离鉴定出三氯乙酸1.86%,顺式-1,2-环己二醇3.56%,庚基氢过氧化物1.66%,Z型-2-十一碳烯5.54%,1-丁氧基-2-乙基己烯4.13%,吡咯并[1,2-A]吡嗪-1,4-二酮6.36%,棕榈酸23.32%,10-十一碳炔酸33.81%,环戊烷十一酸1.11%,2,5-哌嗪,3-苄基-6-异丙基2.95%,2-溴(正)壬烷1.04%,吡咯并[1,2-A]吡嗪-1,4-二酮,六氢-3(苯)5.73%,4-乙基四氢-2H-噻喃1.77%,1,2,2溴二十二烷1.26%,1,1-二氯-2,2,3,3-四甲基环丙烷2.31%,(Z,Z,Z)-6,9,12-十八三烯苯基甲基酯等19种成分。结论:进一步确定了掌叶半夏脂溶性部分的化学成分,为掌叶半夏的抗肿瘤作用深入研究提供参考。

Objective： To analyze the liposoluble components of Pinellia pedatisecta. Method： The liposoluble components of P. pedatisecta were extracted by ethanol ; the extract was abstracted by chloroform, which can get liposoluble components and analyzed by gas chromatography-mass spectrometry （GC-MS）. Result： Nineteen liposoluble components were identified as trichloroacetie acid 1.86% , cis-1, 2-cyclohexanediol 3.56% , heptylhydroperoxide 1.66%, （Z） -2-undecene 5.54%, 1-hexene, 1-butoxy-2-ethyl-4. 13%, pyrrolo [ 1, 2- a ] pyrazine-1 , 4-dione, hexahydro-3- （ 2-methylpropyl ） -6.36% , n-hexadecanoic acid 23.32% , 10- undecynoic acid 33.81% , cyclopentane undecanoic acid 1.11% , 2, 5-piperazinedione, 3-benzyl-6-isopropyl- 2.95%, 2-bromononane 1.04%, pyrrolo [ 1, 2-a] pyrazine-1, 4-dione, hexahydro-3-（ phenylmethy）- 5.73% , 4-ethyltetrahydro-2H-thiopyran 1.77% , docosane, 1, 22-dibromo- 1.26% , cyclopropane, 1, 1- dichloro-2, 2, 3, 3-tetramethyl-2.31%, 6, 9, 12-octadecatrienoic acid, phenylmethyl ester, （Z, Z, Z） 1.00%. Conclusion： The liposoluble components of P. pedatisecta have been further determined to establish the basis for antitumous effect.